Developmental Biology
Anti-SFRP4 Antibody (CAB6409)
- SKU:
- CAB6409
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Description
| Antibody Name: | Anti-SFRP4 Antibody |
| Antibody SKU: | CAB6409 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 19-346 of human SFRP4 (NP_003005.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, 293T, HeLa, HO-8910 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 19-346 of human SFRP4 (NP_003005.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VRGA PCEA VRIP MCRH MPWN ITRM PNHL HHST QENA ILAI EQYE ELVD VNCS AVLR FFLC AMYA PICT LEFL HDPI KPCK SVCQ RARD DCEP LMKM YNHS WPES LACD ELPV YDRG VCIS PEAI VTDL PEDV KWID ITPD MMVQ ERPL DVDC KRLS PDRC KCKK VKPT LATY LSKN YSYV IHAK IKAV QRSG CNEV TTVV DVKE IFKS SSPI PRTQ VPLI TNSS CQCP HILP HQDV LIMC YEWR SRMM LLEN CLVE KWRD QLSK RSIQ WEER LQEQ RRTV QDKK KTAG RTSR SNPP KPKG KPPA PKPA SPKK NIKT RSAQ KRTN PKRV |
| Gene ID: | 6424 |
| Uniprot: | Q6FHJ7 |
| Cellular Location: | Secreted |
| Calculated MW: | 39kDa |
| Observed MW: | 40kDa/55kDa |
| Synonyms: | SFRP4, FRP-4, FRPHE, PYL, sFRP-4 |
| Background: | Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression. |
| UniProt Protein Function: | SFRP4: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling. Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake. Belongs to the secreted frizzled-related protein (sFRP) family. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 7p14.1 Cellular Component: cell surface; cytoplasm; extracellular space; integral to membrane; nucleus Molecular Function:G-protein coupled receptor activity; protein binding; Wnt receptor activity; Wnt-protein binding Biological Process: negative regulation of cell proliferation; negative regulation of transcription factor activity; phosphate ion homeostasis; positive regulation of apoptosis; positive regulation of epidermal cell differentiation; positive regulation of receptor internalization |
| NCBI Summary: | Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q6FHJ7 |
| NCBI GenInfo Identifier: | 61216780 |
| NCBI Gene ID: | 6424 |
| NCBI Accession: | Q6FHJ7.2 |
| UniProt Secondary Accession: | Q6FHJ7,O14877, Q05BG7, Q1ZYW2, Q4G124, Q6FHM0, Q6PD64 B4DYC1, |
| UniProt Related Accession: | Q6FHJ7 |
| Molecular Weight: | 39,827 Da |
| NCBI Full Name: | Secreted frizzled-related protein 4 |
| NCBI Synonym Full Names: | secreted frizzled related protein 4 |
| NCBI Official Symbol: | SFRP4 |
| NCBI Official Synonym Symbols: | PYL; FRP-4; FRPHE; sFRP-4 |
| NCBI Protein Information: | secreted frizzled-related protein 4 |
| UniProt Protein Name: | Secreted frizzled-related protein 4 |
| UniProt Synonym Protein Names: | Frizzled protein, human endometrium; FrpHE |
| Protein Family: | Secreted frizzled-related protein |
| UniProt Gene Name: | SFRP4 |
| UniProt Entry Name: | SFRP4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SFRP4 antibody (CAB6409) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded mouse heart using SFRP4 antibody (CAB6409) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using SFRP4 antibody (CAB6409). Blue: DAPI for nuclear staining. |
View AllClose
Related Products
Human sFRP4 Recombinant Protein (RPES3261)
Growth Factors & Cytokines Recombinant Proteins
system_update_altDatasheetHuman sFRP4 Recombinant Protein SFRP family consists of five secreted glycoproteins in humans acting as extracellular signaling ligands. Each is approximately 300 a
Mouse sFRP4 Recombinant Protein (RPES4983)
system_update_altDatasheetMouse sFRP4 Recombinant Protein SFRP family consists of five secreted glycoproteins in humans acting as extracellular signaling ligands. Each is approximately 300 a