KO Validated Antibodies 1

Anti-SETD7 Antibody (CAB9985)[KO Validated]

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SKU:
CAB9985
Product Type:
Antibody
Applications:
WB
Applications:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-SETD7 Antibody [KO Validated]
Antibody SKU:CAB9985
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-366 of human SETD7 (NP_085151.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-366 of human SETD7 (NP_085151.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MDSD DEMV EEAV EGHL DDDG LPHG FCTV TYSS TDRF EGNF VHGE KNGR GKFF FFDG STLE GYYV DDAL QGQG VYTY EDGG VLQG TYVD GELN GPAQ EYDT DGRL IFKG QYKD NIRH GVCW IYYP DGGS LVGE VNED GEMT GEKI AYVY PDER TALY GKFI DGEM IEGK LATL MSTE EGRP HFEL MPGN SVYH FDKS TSSC ISTN ALLP DPYE SERV YVAE SLIS SAGE GLFS KVAV GPNT VMSF YNGV RITH QEVD SRDW ALNG NTLS LDEE TVID VPEP YNHV SKYC ASLG HKAN HSFT PNCI YDMF VHPR FGPI KCIR TLRA VEAD EELT VAYG YDHS PPGK SGPE APEW YQVE LKAF QATQ QK
Gene ID:80854
Uniprot:Q8WTS6
Cellular Location:Chromosome, Nucleus
Calculated MW:40kDa
Observed MW:48KDa
Synonyms:SETD7, KMT7, SET7, SET7/9, SET9
Background:
UniProt Protein Function:Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation.
UniProt Code:Q8WTS6
NCBI GenInfo Identifier:25091217
NCBI Gene ID:80854
NCBI Accession:Q8WTS6.1
UniProt Secondary Accession:Q8WTS6,Q0VAH3, Q4W5A9, Q9C0E6, B5WWL3,
UniProt Related Accession:Q8WTS6
Molecular Weight:40,721 Da
NCBI Full Name:Histone-lysine N-methyltransferase SETD7
NCBI Synonym Full Names:SET domain containing lysine methyltransferase 7
NCBI Official Symbol:SETD7  
NCBI Official Synonym Symbols:KMT7; SET7; SET9; SET7/9  
NCBI Protein Information:histone-lysine N-methyltransferase SETD7
UniProt Protein Name:Histone-lysine N-methyltransferase SETD7
UniProt Synonym Protein Names:Histone H3-K4 methyltransferase SETD7; H3-K4-HMTase SETD7; Lysine N-methyltransferase 7; SET domain-containing protein 7; SET7/9
Protein Family:Histone-lysine N-methyltransferase
UniProt Gene Name:SETD7  
UniProt Entry Name:SETD7_HUMAN
Anti-SETD7 Polyclonal Antibody [KO Validated] (CAB9985)Western blot analysis of extracts of 293T cells, using [KO Validated] SETD7 antibody (CAB9985) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
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