Metabolism Antibodies 2
Anti-SERPINH1 Antibody (CAB2517)
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- SKU:
- CAB2517
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-SERPINH1 Antibody |
| Antibody SKU: | CAB2517 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 139-418 of human SERPINH1 (NP_001226.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, M21, HeLa, U-251MG, THP-1 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 139-418 of human SERPINH1 (NP_001226.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SVSF ADDF VRSS KQHY NCEH SKIN FRDK RSAL QSIN EWAA QTTD GKLP EVTK DVER TDGA LLVN AMFF KPHW DEKF HHKM VDNR GFMV TRSY TVGV MMMH RTGL YNYY DDEK EKLQ IVEM PLAH KLSS LIIL MPHH VEPL ERLE KLLT KEQL KIWM GKMQ KKAV AISL PKGV VEVT HDLQ KHLA GLGL TEAI DKNK ADLS RMSG KKDL YLAS VFHA TAFE LDTD GNPF DQDI YGRE ELRS PKLF YADH PFIF LVRD TQSG SLLF IGRL VRPK GDKM RDEL |
| Gene ID: | 871 |
| Uniprot: | P50454 |
| Cellular Location: | Endoplasmic reticulum lumen |
| Calculated MW: | 46kDa |
| Observed MW: | 46kDa |
| Synonyms: | SERPINH1, AsTP3, CBP1, CBP2, HSP47, OI10, PIG14, PPROM, RA-A47, SERPINH2, gp46, serpin H1 |
| Background: | This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum and plays a role in collagen biosynthesis as a collagen-specific molecular chaperone. Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. Expression of this gene may be a marker for cancer, and nucleotide polymorphisms in this gene may be associated with preterm birth caused by preterm premature rupture of membranes. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9. |
| UniProt Protein Function: | SERPINH1: Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen. Defects in SERPINH1 are the cause of osteogenesis imperfecta type 10 (OI10). A connective tissue disorder characterized by bone fragility, low bone mass, bowing of limbs due to multiple fractures, short limb dwarfism and blue sclerae. Belongs to the serpin family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 11q13.5 Cellular Component: extracellular space; endoplasmic reticulum; endoplasmic reticulum lumen; ER-Golgi intermediate compartment Molecular Function:serine-type endopeptidase inhibitor activity; collagen binding; unfolded protein binding Biological Process: extracellular matrix organization and biogenesis; collagen fibril organization; protein maturation; collagen biosynthetic process; response to unfolded protein Disease: Osteogenesis Imperfecta, Type X; Preterm Premature Rupture Of The Membranes |
| NCBI Summary: | This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum and plays a role in collagen biosynthesis as a collagen-specific molecular chaperone. Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. Expression of this gene may be a marker for cancer, and nucleotide polymorphisms in this gene may be associated with preterm birth caused by preterm premature rupture of membranes. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, May 2011] |
| UniProt Code: | P50454 |
| NCBI GenInfo Identifier: | 20141241 |
| NCBI Gene ID: | 871 |
| NCBI Accession: | P50454.2 |
| UniProt Secondary Accession: | P50454,P29043, Q5XPB4, Q6NSJ6, Q8IY96, Q9NP88, B3KVJ3 |
| UniProt Related Accession: | P50454 |
| Molecular Weight: | 418 |
| NCBI Full Name: | Serpin H1 |
| NCBI Synonym Full Names: | serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) |
| NCBI Official Symbol: | SERPINH1 |
| NCBI Official Synonym Symbols: | CBP1; CBP2; OI10; gp46; AsTP3; HSP47; PIG14; PPROM; RA-A47; SERPINH2 |
| NCBI Protein Information: | serpin H1; colligin-1; colligin-2; 47 kDa heat shock protein; arsenic-transactivated protein 3; rheumatoid arthritis antigen A-47; cell proliferation-inducing gene 14 protein; rheumatoid arthritis-related antigen RA-A47; serine (or cysteine) proteinase in |
| UniProt Protein Name: | Serpin H1 |
| UniProt Synonym Protein Names: | 47 kDa heat shock protein; Arsenic-transactivated protein 3; AsTP3; Cell proliferation-inducing gene 14 protein; Collagen-binding protein; Colligin; Rheumatoid arthritis-related antigen RA-A47 |
| UniProt Gene Name: | SERPINH1 |
| UniProt Entry Name: | SERPH_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SERPINH1 antibody (CAB2517) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of HeLa cells using SERPINH1 antibody (CAB2517). Blue: DAPI for nuclear staining. |
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