Cell Biology Antibodies 2

Anti-SERPINE1 Antibody (CAB12102)

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SKU:
CAB12102
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-SERPINE1 Antibody
Antibody SKU:CAB12102
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 24-158 of human SERPINE1 (NP_000593.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:HT-1080, SH-SY5Y, Mouse liver, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 24-158 of human SERPINE1 (NP_000593.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:VHHP PSYV AHLA SDFG VRVF QQVA QASK DRNV VFSP YGVA SVLA MLQL TTGG ETQQ QIQA AMGF KIDD KGMA PALR HLYK ELMG PWNK DEIS TTDA IFVQ RDLK LVQG FMPH FFRL FRST VKQV DFSE VERA RFI
Gene ID:5054
Uniprot:P05121
Cellular Location:Secreted
Calculated MW:43kDa/45kDa
Observed MW:45kDa
Synonyms:PAI, PAI-1, PAI1, PLANH1, SERPINE1
Background:This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:SERPINE1: a secreted protein that acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. Belongs to the serpin family. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation. Plasma levels of PAI-1 and VCAM-1 together may be useful in predicting post-operative recurrence in patients with colorectal cancer.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: extracellular matrix; extracellular space; extracellular region; plasma membrane

Molecular Function:serine-type endopeptidase inhibitor activity; protein binding; protease binding; receptor binding

Biological Process: circadian rhythm; transcription initiation from RNA polymerase II promoter; platelet activation; extracellular matrix organization and biogenesis; positive regulation of blood coagulation; transcription, DNA-dependent; negative regulation of blood coagulation; negative regulation of smooth muscle cell migration; defense response to Gram-negative bacterium; positive regulation of receptor-mediated endocytosis; regulation of cell proliferation; positive regulation of interleukin-8 production; fibrinolysis; positive regulation of angiogenesis; platelet degranulation; negative regulation of fibrinolysis; transforming growth factor beta receptor signaling pathway; gene expression; positive regulation of transcription from RNA polymerase II promoter; regulation of receptor activity; angiogenesis; chronological cell aging; blood coagulation; negative regulation of cell adhesion mediated by integrin; negative regulation of cell migration; positive regulation of inflammatory response

Disease: Plasminogen Activator Inhibitor-1 Deficiency

NCBI Summary:This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
UniProt Code:P05121
NCBI GenInfo Identifier:129576
NCBI Gene ID:5054
NCBI Accession:P05121.1
UniProt Secondary Accession:P05121,B7Z4S0, F8WD53,
UniProt Related Accession:P05121
Molecular Weight:43,404 Da
NCBI Full Name:Plasminogen activator inhibitor 1
NCBI Synonym Full Names:serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
NCBI Official Symbol:SERPINE1  
NCBI Official Synonym Symbols:PAI; PAI1; PAI-1; PLANH1  
NCBI Protein Information:plasminogen activator inhibitor 1; serpin E1; endothelial plasminogen activator inhibitor; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
UniProt Protein Name:Plasminogen activator inhibitor 1
UniProt Synonym Protein Names:Endothelial plasminogen activator inhibitor; Serpin E1
UniProt Gene Name:SERPINE1  
UniProt Entry Name:PAI1_HUMAN
Anti-SERPINE1 Antibody (CAB12102)Western blot analysis of extracts of various cell lines, using SERPINE1 antibody (CAB12102) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 40s.
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