Developmental Biology

Anti-Semaphorin-4C Antibody (CAB8225)

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SKU:
CAB8225
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Developmental Biology

Description

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Antibody Name:SEMA4C Rabbit Polyclonal Antibody
Antibody SKU:CAB8225
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 749-833 of human SEMA4C (NP_060259.4).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:MCF7, BT-474, Mouse heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 749-833 of human SEMA4C (NP_060259.4).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:HARC QPGG GPPS PPPG IPGQ PLPS PTRL HLGG GRNS NANG YVRL QLGG EDRG GLGH PLPE LADE LRRK LQQR QPLP DSNP EESS V
Gene ID:54910
Uniprot:Q9C0C4
Cellular Location:Cell junction, Cytoplasmic vesicle, Single-pass type I membrane protein, postsynaptic cell membrane, postsynaptic density, secretory vesicle, synapse, synaptic vesicle membrane
Calculated MW:92kDa
Observed MW:110kDa
Synonyms:SEMA4C, M-SEMA-F, SEMACL1, SEMAF, SEMAI
Background:
UniProt Protein Function:SEMA4C: Cell surface receptor for PLXNB2 that plays an important role in cell-cell signaling. PLXNB2 binding promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal brain development, axon guidance and cell migration. Probable signaling receptor which may play a role in myogenic differentiation through activation of the stress-activated MAPK cascade. Belongs to the semaphorin family.
UniProt Protein Details:

Protein type:Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q11.2

Cellular Component: extracellular space; membrane; postsynaptic density; synaptic vesicle membrane

Molecular Function:chemorepellent activity; protein binding; semaphorin receptor binding

Biological Process: cell migration in hindbrain; muscle cell differentiation; negative chemotaxis; negative regulation of axon extension involved in axon guidance; neural crest cell migration; neural tube closure; positive regulation of cell migration; positive regulation of stress-activated MAPK cascade

UniProt Code:Q9C0C4
NCBI GenInfo Identifier:47606208
NCBI Gene ID:54910
NCBI Accession:Q9C0C4.2
UniProt Secondary Accession:Q9C0C4,Q32MJ3, Q7Z5X0,
UniProt Related Accession:Q9C0C4
Molecular Weight:92,623 Da
NCBI Full Name:Semaphorin-4C
NCBI Synonym Full Names:semaphorin 4C
NCBI Official Symbol:SEMA4C  
NCBI Official Synonym Symbols:SEMAF; SEMAI; SEMACL1; M-SEMA-F  
NCBI Protein Information:semaphorin-4C
UniProt Protein Name:Semaphorin-4C
Protein Family:Semaphorin
UniProt Gene Name:SEMA4C  
UniProt Entry Name:SEM4C_HUMAN
Anti-Semaphorin-4C Polyclonal Antibody (CAB8225)Western blot analysis of extracts of various cell lines, using SEMA4C antibody (CAB8225) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-Semaphorin-4C Polyclonal Antibody (CAB8225)Immunohistochemistry of paraffin-embedded human lung cancer using SEMA4C antibody (CAB8225) at dilution of 1:100 (40x lens).
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