Developmental Biology
Anti-SEMA5A Antibody (CAB14817)
- SKU:
- CAB14817
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Description
| Antibody Name: | Anti-SEMA5A Antibody |
| Antibody SKU: | CAB14817 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 330-500 of human SEMA5A (NP_003957.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse |
| Positive Samples: | Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 330-500 of human SEMA5A (NP_003957.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AFSG PFKY QENS RSAW LPYP NPNP HFQC GTVD QGLY VNLT ERNL QDAQ KFIL MHEV VQPV TTVP SFME DNSR FSHV AVDV VQGR EALV HIIY LATD YGTI KKVR VPLN QTSS SCLL EEIE LFPE RRRE PIRS LQIL HSQS VLFV GLRE HVVK IPLK RCQF YRTR STCI GAQ |
| Gene ID: | 9037 |
| Uniprot: | Q13591 |
| Cellular Location: | Membrane, Single-pass type I membrane protein |
| Calculated MW: | 120kDa |
| Observed MW: | 150kDa |
| Synonyms: | SEMA5A, SEMAF, semF |
| Background: | This gene belongs to the semaphorin gene family that encodes membrane proteins containing a semaphorin domain and several thrombospondin type-1 repeats. Members of this family are involved in axonal guidance during neural development. This gene has been implicated as an autism susceptibility gene. |
| UniProt Protein Function: | SEMA5A: a single-pass type I membrane protein that may act as positive axonal guidance cues. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 5p15.2 Cellular Component: integral to membrane; membrane; plasma membrane Molecular Function:chemorepellent activity; heparan sulfate proteoglycan binding; semaphorin receptor binding; syndecan binding Biological Process: axonal fasciculation; blood vessel endothelial cell proliferation during sprouting angiogenesis; cell adhesion; cell-cell signaling; diencephalon development; negative regulation of axon extension involved in axon guidance; negative regulation of cell adhesion; nervous system development; neural crest cell migration; positive chemotaxis; positive regulation of actin filament depolymerization; positive regulation of angiogenesis; positive regulation of axon extension involved in axon guidance; positive regulation of cell migration; positive regulation of endothelial cell proliferation; positive regulation of protein kinase B signaling cascade |
| NCBI Summary: | This gene belongs to the semaphorin gene family that encodes membrane proteins containing a semaphorin domain and several thrombospondin type-1 repeats. Members of this family are involved in axonal guidance during neural development. This gene has been implicated as an autism susceptibility gene.[provided by RefSeq, Jan 2010] |
| UniProt Code: | Q13591 |
| NCBI GenInfo Identifier: | 109939725 |
| NCBI Gene ID: | 9037 |
| NCBI Accession: | Q13591.3 |
| UniProt Secondary Accession: | Q13591,O60408, Q1RLL9, D3DTC6, |
| UniProt Related Accession: | Q13591 |
| Molecular Weight: | 120,615 Da |
| NCBI Full Name: | Semaphorin-5A |
| NCBI Synonym Full Names: | semaphorin 5A |
| NCBI Official Symbol: | SEMA5A |
| NCBI Official Synonym Symbols: | semF; SEMAF |
| NCBI Protein Information: | semaphorin-5A |
| UniProt Protein Name: | Semaphorin-5A |
| UniProt Synonym Protein Names: | Semaphorin-F; Sema F |
| Protein Family: | Semaphorin |
| UniProt Gene Name: | SEMA5A |
| UniProt Entry Name: | SEM5A_HUMAN |
 | Western blot analysis of extracts of mouse liver, using SEMA5A antibody (CAB14817) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min. |
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