Developmental Biology
Anti-SEMA4G Antibody (CAB15492)
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- SKU:
- CAB15492
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Description
| Antibody Name: | Anti-SEMA4G Antibody |
| Antibody SKU: | CAB15492 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 470-560 of human SEMA4G (NP_001190173.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:200 - 1:2000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse brain, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 470-560 of human SEMA4G (NP_001190173.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ETQV FRES QSVE NLVI SLLQ HSLY VGAP SGVI QLPL SSCS RYRS CYDC ILAR DPYC GWDP GTHA CAAA TTIA NRSQ GSRT ALIQ DIER GNR |
| Gene ID: | 57715 |
| Uniprot: | Q9NTN9 |
| Cellular Location: | Cell membrane, Single-pass type I membrane protein |
| Calculated MW: | 77kDa/91kDa/92kDa |
| Observed MW: | 105kDa |
| Synonyms: | SEMA4G |
| Background: | Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes, some (if not all) containing plexin proteins. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | SEMA4G: a single-pass type I membrane protein that may play a role in axon guidance. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 10q24.31 Cellular Component: extracellular space Molecular Function:chemorepellent activity; protein binding; semaphorin receptor binding Biological Process: negative chemotaxis; negative regulation of axon extension involved in axon guidance; neural crest cell migration; positive regulation of cell migration |
| NCBI Summary: | Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes, some (if not all) containing plexin proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011] |
| UniProt Code: | Q9NTN9 |
| NCBI GenInfo Identifier: | 13633937 |
| NCBI Gene ID: | 57715 |
| NCBI Accession: | Q9NTN9.1 |
| UniProt Secondary Accession: | Q9NTN9,Q58EY1, Q9HCF3, A1A5C6, A6NJY8, |
| UniProt Related Accession: | Q9NTN9 |
| Molecular Weight: | 77,577 Da |
| NCBI Full Name: | Semaphorin-4G |
| NCBI Synonym Full Names: | semaphorin 4G |
| NCBI Official Symbol: | SEMA4G |
| NCBI Protein Information: | semaphorin-4G |
| UniProt Protein Name: | Semaphorin-4G |
| Protein Family: | Semaphorin |
| UniProt Gene Name: | SEMA4G |
| UniProt Entry Name: | SEM4G_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SEMA4G antibody (CAB15492) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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