Developmental Biology
Anti-SEMA4D Antibody (CAB10136)
- SKU:
- CAB10136
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Description
| Antibody Name: | Anti-SEMA4D Antibody |
| Antibody SKU: | CAB10136 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 753-862 of human SEMA4D (NP_006369.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:200 - 1:1000 |
| Reactivity: | Human |
| Positive Samples: | 293T |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 753-862 of human SEMA4D (NP_006369.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | YNCY KGYL PRQC LKFR SALL IGKK KPKS DFCD REQS LKET LVEP GSFS QQNG EHPK PALD TGYE TEQD TITS KVPT DRED SQRI DDLS ARDK PFDV KCEL KFAD SDAD GD |
| Gene ID: | 10507 |
| Uniprot: | Q92854 |
| Cellular Location: | Cell membrane, Single-pass type I membrane protein |
| Calculated MW: | 82kDa/96kDa |
| Observed MW: | 150kDa |
| Synonyms: | SEMA4D, C9orf164, CD100, M-sema-G, SEMAJ, coll-4 |
| Background: |
| UniProt Protein Function: | SEMA4D: a single-pass type I membrane protein that my play a functional role in the immune system, as well as in the nervous system. Induces B-cells to aggregate and improves their viability in vitro. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 9q22.2 Cellular Component: extracellular space; integral to plasma membrane; plasma membrane Molecular Function:chemorepellent activity; protein binding; receptor activity; receptor binding; semaphorin receptor binding; transmembrane receptor activity Biological Process: axon guidance; cell adhesion; immune response; negative chemotaxis; negative regulation of apoptosis; negative regulation of axon extension involved in axon guidance; negative regulation of cell adhesion; negative regulation of osteoblast differentiation; negative regulation of peptidyl-tyrosine phosphorylation; negative regulation of transcription from RNA polymerase II promoter; neural crest cell migration; positive regulation of cell migration; positive regulation of collateral sprouting; positive regulation of GTPase activity; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein amino acid phosphorylation; regulation of cell projection organization and biogenesis; regulation of cell shape; regulation of dendrite morphogenesis |
| UniProt Code: | Q92854 |
| NCBI GenInfo Identifier: | 8134701 |
| NCBI Gene ID: | 10507 |
| NCBI Accession: | Q92854.1 |
| UniProt Secondary Accession: | Q92854,Q7Z5S4, Q8N8B0, B2RPM6, |
| UniProt Related Accession: | Q92854 |
| Molecular Weight: | 82,164 Da |
| NCBI Full Name: | Semaphorin-4D |
| NCBI Synonym Full Names: | semaphorin 4D |
| NCBI Official Symbol: | SEMA4D |
| NCBI Official Synonym Symbols: | CD100; SEMAJ; coll-4; C9orf164; M-sema-G |
| NCBI Protein Information: | semaphorin-4D |
| UniProt Protein Name: | Semaphorin-4D |
| UniProt Synonym Protein Names: | A8; BB18; GR3; CD_antigen: CD100 |
| Protein Family: | Semaphorin |
| UniProt Gene Name: | SEMA4D |
| UniProt Entry Name: | SEM4D_HUMAN |
 | Western blot analysis of extracts of 293T cells, using SEMA4D antibody (CAB10136) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 10s. |
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