Developmental Biology
Anti-SEMA3C Antibody (CAB15386)
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- SKU:
- CAB15386
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Description
| Antibody Name: | Anti-SEMA3C Antibody |
| Antibody SKU: | CAB15386 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 21-330 of human SEMA3C (NP_006370.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:200 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse small intestine, Rat small intestine |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 21-330 of human SEMA3C (NP_006370.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GSSQ PQAR VYLT FDEL RETK TSEY FSLS HHPL DYRI LLMD EDQD RIYV GSKD HILS LNIN NISQ EALS VFWP ASTI KVEE CKMA GKDP THGC GNFV RVIQ TFNR THLY VCGS GAFS PVCT YLNR GRRS EDQV FMID SKCE SGKG RCSF NPNV NTVS VMIN EELF SGMY IDFM GTDA AIFR SLTK RNAV RTDQ HNSK WLSE PMFV DAHV IPDG TDPN DAKV YFFF KEKL TDNN RSTK QIHS MIAR ICPN DTGG LRSL VNKW TTFL KARL VCSV TDED GPET HFDE LEDV FLLE TDNP RTTL VYGI FTTS SS |
| Gene ID: | 10512 |
| Uniprot: | Q99985 |
| Cellular Location: | Secreted |
| Calculated MW: | 26kDa/85kDa |
| Observed MW: | 85kDa |
| Synonyms: | SEMA3C, SEMAE, SemE |
| Background: |
| UniProt Protein Function: | SEMA3C: a secreted protein that may be involved in cell survival mechanisms. Expressed intensely in the heart, skeletal muscle, colon, small intestine, ovary, testis, and prostate. Faint expression ubiquitously among other organs, including brain. Binds with high affinity to the receptor NRP2. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 7q21-q31 Cellular Component: extracellular space; membrane Molecular Function:receptor activity; semaphorin receptor binding Biological Process: neural tube development; response to drug; axon guidance; limb bud formation; somitogenesis; blood vessel remodeling; immune response; neural crest cell migration; post-embryonic development |
| NCBI Summary: | This gene encodes a secreted glycoprotein that belongs to the semaphorin class 3 family of neuronal guidance cues. The encoded protein contains an N-terminal sema domain, integrin and immunoglobulin-like domains, and a C-terminal basic domain. Homodimerization and proteolytic cleavage of the C-terminal propeptide are necessary for the function of the encoded protein. It binds a neuropilin co-receptor before forming a heterotrimeric complex with an associated plexin. An increase in the expression of this gene correlates with an increase in cancer cell invasion and adhesion. Naturally occurring mutations in this gene are associated with Hirschsprung disease. [provided by RefSeq, May 2017] |
| UniProt Code: | Q99985 |
| NCBI GenInfo Identifier: | 8134685 |
| NCBI Gene ID: | 10512 |
| NCBI Accession: | Q99985.2 |
| UniProt Related Accession: | Q99985 |
| Molecular Weight: | ~ 85kDa |
| NCBI Full Name: | Semaphorin-3C |
| NCBI Synonym Full Names: | semaphorin 3C |
| NCBI Official Symbol: | SEMA3C |
| NCBI Official Synonym Symbols: | SemE; SEMAE |
| NCBI Protein Information: | semaphorin-3C |
| UniProt Protein Name: | Semaphorin-3C |
| UniProt Synonym Protein Names: | Semaphorin-E; Sema E |
| Protein Family: | Semaphorin |
| UniProt Gene Name: | SEMA3C |
| UniProt Entry Name: | SEM3C_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SEMA3C antibody (CAB15386) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of L929 cells using SEMA3C antibody (CAB15386) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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