Antibody Name:	Anti-SELENBP1 Antibody [KO Validated]
Antibody SKU:	CAB1222
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 243-472 of human SELENBP1 (NP_003935.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	MCF7, Mouse liver, Mouse kideny

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 243-472 of human SELENBP1 (NP_003935.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SLKD GLIP LEIR FLHN PDAA QGFV GCAL SSTI QRFY KNEG GTWS VEKV IQVP PKKV KGWL LPEM PGLI TDIL LSLD DRFL YFSN WLHG DLRQ YDIS DPQR PRLT GQLF LGGS IVKG GPVQ VLED EELK SQPE PLVV KGKR VAGG PQMI QLSL DGKR LYIT TSLY SAWD KQFY PDLI REGS VMLQ VDVD TVKG GLKL NPNF LVDF GKEP LGPA LAHE LRYP GGDC SSDI WI
Gene ID:	8991
Uniprot:	Q13228
Cellular Location:	Cytoplasm, Membrane, Nucleus, Peripheral membrane protein, cytosol
Calculated MW:	45kDa/52kDa/56kDa
Observed MW:	55kDa

Synonyms:

SELENBP1, HEL-S-134P, LPSB, SBP56, SP56, hSBP

Background:

This gene encodes a member of the selenium-binding protein family. Selenium is an essential nutrient that exhibits potent anticarcinogenic properties, and deficiency of selenium may cause certain neurologic diseases. The effects of selenium in preventing cancer and neurologic diseases may be mediated by selenium-binding proteins, and decreased expression of this gene may be associated with several types of cancer. The encoded protein may play a selenium-dependent role in ubiquitination/deubiquitination-mediated protein degradation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

UniProt Protein Function:	SELENBP1: Selenium-binding protein which may be involved in the sensing of reactive xenobiotics in the cytoplasm. May be involved in intra-Golgi protein transport. Belongs to the selenium-binding protein family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 1q21.3 Cellular Component: extracellular space; membrane; nucleolus; cytosol Molecular Function:selenium binding; protein binding Biological Process: protein transport
NCBI Summary:	This gene encodes a member of the selenium-binding protein family. Selenium is an essential nutrient that exhibits potent anticarcinogenic properties, and deficiency of selenium may cause certain neurologic diseases. The effects of selenium in preventing cancer and neurologic diseases may be mediated by selenium-binding proteins, and decreased expression of this gene may be associated with several types of cancer. The encoded protein may play a selenium-dependent role in ubiquitination/deubiquitination-mediated protein degradation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Apr 2012]
UniProt Code:	Q13228
NCBI GenInfo Identifier:	148840437
NCBI Gene ID:	8991
NCBI Accession:	Q13228.2
UniProt Secondary Accession:	Q13228,Q49AQ8, Q96GX7, A6NML9, A6PVW9, B2RDR3, B4DKP6 B4E1F3,
UniProt Related Accession:	Q13228
Molecular Weight:	472
NCBI Full Name:	Selenium-binding protein 1
NCBI Synonym Full Names:	selenium binding protein 1
NCBI Official Symbol:	SELENBP1
NCBI Official Synonym Symbols:	LPSB; SP56; hSBP; SBP56; HEL-S-134P
NCBI Protein Information:	selenium-binding protein 1; 56 kDa selenium-binding protein; epididymis secretory sperm binding protein Li 134P
UniProt Protein Name:	Selenium-binding protein 1
UniProt Synonym Protein Names:	56 kDa selenium-binding protein; SBP56; SP56
Protein Family:	Selenium-binding protein
UniProt Gene Name:	SELENBP1
UniProt Entry Name:	SBP1_HUMAN

	Western blot analysis of extracts of various cell lines, using SELENBP1 antibody (CAB1222) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020).
	Western blot analysis of extracts from normal (control) and SELENBP1 knockout (KO) HeLa cells, using SELENBP1 antibody (CAB1222) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded human colon using SELENBP1 Antibody (CAB1222) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using SELENBP1 antibody (CAB1222). Blue: DAPI for nuclear staining.