Anti-SDHC Antibody (CAB21040)
- SKU:
- CAB21040
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
Description
| Antibody Name: | Anti-SDHC Antibody (CAB21040) |
| Antibody SKU: | CAB21040 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting, Immunohistochemistry, Immunofluorescence |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SDHC (NP_002992.1). |
| Application: | Western blotting, Immunohistochemistry, Immunofluorescence |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, HepG2, Mouse liver, Rat liver |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SDHC (NP_002992.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAL LLRH VGRH CLRA HFSP QLCI RNAV PLGT TAKE EMER FWNK NIGS NRPL SPHI TIYS WSLP MAMS ICHR GTGI ALSA GVSL FGMS ALLL PGNF ESYL |
| Cellular Location: | Mitochondrion inner membrane, Multi-pass membrane protein |
| Calculated MW: | 12kDa/14kDa/16kDa/18kDa |
| Observed MW: | 12KDa |
| Synonyms: | SDHC, CYB560, CYBL, PGL3, QPS1, SDH3 |
| Background: | This gene encodes one of four nuclear-encoded subunits that comprise succinate dehydrogenase, also known as mitochondrial complex II, a key enzyme complex of the tricarboxylic acid cycle and aerobic respiratory chains of mitochondria. The encoded protein is one of two integral membrane proteins that anchor other subunits of the complex, which form the catalytic core, to the inner mitochondrial membrane. There are several related pseudogenes for this gene on different chromosomes. Mutations in this gene have been associated with paragangliomas. Alternatively spliced transcript variants have been described. |
 | Immunohistochemistry of paraffin-embedded rat kidney using SDHC Rabbit pAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Western blot analysis of extracts of various cell lines, using SDHC antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. |
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