Antibody Name:	Anti-SCD Antibody [KO Validated]
Antibody SKU:	CAB16429
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human SCD (NP_005054.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse liver, Rat liver

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human SCD (NP_005054.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LDLS DLEA EKLV MFQR RYYK PGLL MMCF ILPT LVPW YFWG ETFQ NSVF VATF LRYA VVLN ATWL VNSA AHLF GYRP YDKN ISPR ENIL VSLG AVGE GFHN YHHS FPYD YSAS EYRW HINF TTFF IDCM AALG LAYD RKKV SKAA ILAR IKRT GDGN YKSG
Gene ID:	6319
Uniprot:	O00767
Cellular Location:	Endoplasmic reticulum membrane, Multi-pass membrane protein
Calculated MW:	41kDa
Observed MW:	37KDa

Synonyms:

SCD, FADS5, MSTP008, SCD1, SCDOS, hSCD1

Background:

This gene encodes an enzyme involved in fatty acid biosynthesis, primarily the synthesis of oleic acid. The protein belongs to the fatty acid desaturase family and is an integral membrane protein located in the endoplasmic reticulum. Transcripts of approximately 3.9 and 5.2 kb, differing only by alternative polyadenlyation signals, have been detected. A gene encoding a similar enzyme is located on chromosome 4 and a pseudogene of this gene is located on chromosome 17.

UniProt Protein Function:	SCD: Terminal component of the liver microsomal stearyl-CoA desaturase system, that utilizes O(2) and electrons from reduced cytochrome b5 to catalyze the insertion of a double bond into a spectrum of fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA. Belongs to the fatty acid desaturase family.
UniProt Protein Details:	Protein type:EC 1.14.19.1; Endoplasmic reticulum; Lipid Metabolism - unsaturated fatty acid biosynthesis; Membrane protein, integral; Membrane protein, multi-pass; Oxidoreductase Chromosomal Location of Human Ortholog: 10q24.31 Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral to membrane; membrane; nucleolus Molecular Function:iron ion binding; oxidoreductase activity; stearoyl-CoA 9-desaturase activity Biological Process: unsaturated fatty acid biosynthetic process
NCBI Summary:	This gene encodes an enzyme involved in fatty acid biosynthesis, primarily the synthesis of oleic acid. The protein belongs to the fatty acid desaturase family and is an integral membrane protein located in the endoplasmic reticulum. Transcripts of approximately 3.9 and 5.2 kb, differing only by alternative polyadenlyation signals, have been detected. A gene encoding a similar enzyme is located on chromosome 4 and a pseudogene of this gene is located on chromosome 17. [provided by RefSeq, Sep 2015]
UniProt Code:	O00767
NCBI GenInfo Identifier:	21431730
NCBI Gene ID:	6319
NCBI Accession:	O00767.2
UniProt Secondary Accession:	O00767,Q16150, Q53GR9, Q5W037, Q5W038, Q6GSS4, Q96KF6 Q9BS07, Q9Y695, B2R5U0, D3DR68,
UniProt Related Accession:	O00767
Molecular Weight:	41,523 Da
NCBI Full Name:	Acyl-CoA desaturase
NCBI Synonym Full Names:	stearoyl-CoA desaturase
NCBI Official Symbol:	SCD
NCBI Official Synonym Symbols:	SCD1; FADS5; SCDOS; hSCD1; MSTP008
NCBI Protein Information:	acyl-CoA desaturase
UniProt Protein Name:	Acyl-CoA desaturase
UniProt Synonym Protein Names:	Delta(9)-desaturase; Delta-9 desaturase; Fatty acid desaturase; Stearoyl-CoA desaturase
Protein Family:	Acyl-CoA desaturase
UniProt Gene Name:	SCD

	Western blot analysis of extracts from normal (control) and SCD knockout (KO) HeLa cells, using SCD antibody (CAB16429) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Western blot analysis of extracts of various cell lines, using SCD antibody (CAB16429) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded rat brain using [KO Validated] SCD Rabbit pAb (CAB16429) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using [KO Validated] SCD Rabbit pAb (CAB16429) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U-2 OS cells using SCD Polyclonal Antibody (CAB16429) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.