Antibody Name:	Anti-SAV1 Antibody [KO Validated]
Antibody SKU:	CAB18667
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human SAV1.

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, 293T

Immunogen:	Recombinant protein of human SAV1.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	60485
Uniprot:	Q9H4B6
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	44kDa
Observed MW:	45kDa

	Western blot analysis of extracts from normal (control) and [KO Validated] SAV1 knockout (KO) HeLa cells, using [KO Validated] SAV1 antibody (CAB18667) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Western blot analysis of extracts of 293T cells, using [KO Validated] SAV1 Rabbit pAb (CAB18667) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded Mouse kidney using [KO Validated] SAV1 Rabbit pAb (CAB18667) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Rat kidney using [KO Validated] SAV1 Rabbit pAb (CAB18667) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Human colon using [KO Validated] SAV1 Rabbit pAb (CAB18667) at dilution of 1:100 (40x lens).

Synonyms:

SAV1, SAV, WW45, WWP4, protein salvador homolog 1

Background:

WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein with two WW domains, a SARAH domain, and a coiled-coil region and is ubiquitously expressed in adult tissues. This protein binds to MST1 (mammalian sterile 20-like kinase 1) and promotes MST1-induced apoptosis. It has also been shown to bind to HAX1 (hematopoietic cell-specific protein 1 (HS1)-associated protein X-1) and to attenuate the anti-apoptotic effects of HAX1. Studies in human and mouse suggest this gene acts as a tumor suppressor.

UniProt Protein Function:	SAV1: Regulator of STK3/MST2 and STK4/MST1 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. SAV1 is required for STK3/MST2 and STK4/MST1 activation and promotes cell-cycle exit and terminal differentiation in developing epithelial tissues. Plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosomes, and its ability to phosphorylate CROCC and CEP250. In conjunction with STK3/MST2, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation. Homodimer. Stabilized through interaction with STK3/MST2 or STK4/MST1. Interacts (via SARAH domain) with isoform 1 of NEK2. Interacts with ESR1 only in the presence of STK3/MST2. Interacts with WTIP and AJUBA. Ubiquitously expressed in adult tissues with highest expression in the pancreas, aorta and interventricular septum and lowest expression in skeletal muscle. Expression was higher in fetal than in the adult heart. Expressed in various cell lines.
UniProt Protein Details:	Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 14q13-q23 Cellular Component: cytoplasm; cytosol; nucleus Molecular Function:protein binding
NCBI Summary:	WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein with two WW domains, a SARAH domain, and a coiled-coil region and is ubiquitously expressed in adult tissues. This protein binds to MST1 (mammalian sterile 20-like kinase 1) and promotes MST1-induced apoptosis. It has also been shown to bind to HAX1 (hematopoietic cell-specific protein 1 (HS1)-associated protein X-1) and to attenuate the anti-apoptotic effects of HAX1. Studies in human and mouse suggest this gene acts as a tumor suppressor. [provided by RefSeq, Aug 2012]
UniProt Code:	Q9H4B6
NCBI GenInfo Identifier:	32699681
NCBI Gene ID:	60485
NCBI Accession:	Q9H4B6.2
UniProt Secondary Accession:	Q9H4B6,Q6IA58, Q9H949, Q9HAK9, A8K4B8, D3DSB6,
UniProt Related Accession:	Q9H4B6
Molecular Weight:	44,634 Da
NCBI Full Name:	Protein salvador homolog 1
NCBI Synonym Full Names:	salvador family WW domain containing protein 1
NCBI Official Symbol:	SAV1
NCBI Official Synonym Symbols:	SAV; WW45; WWP4
NCBI Protein Information:	protein salvador homolog 1
UniProt Protein Name:	Protein salvador homolog 1
UniProt Synonym Protein Names:	45 kDa WW domain protein; hWW45
Protein Family:	Protein salvador
UniProt Gene Name:	SAV1
UniProt Entry Name:	SAV1_HUMAN