Name: Anti-SATB1 Antibody (CAB5800)
Brand: Immunology Antibodies 2
SKU: CAB5800
Availability: OutOfStock

Antibody Name:	Anti-SATB1 Antibody
Antibody SKU:	CAB5800
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 484-763 of human SATB1 (NP_001124482.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 484-763 of human SATB1 (NP_001124482.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	NGKP ENNT MNIN ASIY DEIQ QEMK RAKV SQAL FAKV AATK SQGW LCEL LRWK EDPS PENR TLWE NLSM IRRF LSLP QPER DAIY EQES NAVH HHGD RPPH IIHV PAEQ IQQQ QQQQ QQQQ QQQQ APPP PQPQ QQPQ TGPR LPPR QPTV ASPA ESDE ENRQ KTRP RTKI SVEA LGIL QSFI QDVG LYPD EEAI QTLS AQLD LPKY TIIK FFQN QRYY LKHH GKLK DNSG LEVD VAEY KEEE LLKD LEES VQDK NTNT LFSV KLEE ELSV EGNT DINT DLKD
Gene ID:	6304
Uniprot:	Q01826
Cellular Location:	Nucleus, Nucleus matrix, PML body
Calculated MW:	85kDa/89kDa
Observed MW:	100KDa

Synonyms:	SATB1
Background:	This gene encodes a matrix protein which binds nuclear matrix and scaffold-associating DNAs through a unique nuclear architecture. The protein recruits chromatin-remodeling factors in order to regulate chromatin structure and gene expression.

UniProt Protein Function:	SATB1: Crucial silencing factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma. Binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence (CSBS), at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcriptional repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes (e.g. PML at the MHC-I locus) and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Modulates genes that are essential in the maturation of the immune T-cell CD8SP from thymocytes. Required for the switching of fetal globin species, and beta- and gamma-globin genes regulation during erythroid differentiation. Plays a role in chromatin organization and nuclear architecture during apoptosis. Interacts with the unique region (UR) of cytomegalovirus (CMV). Alu-like motifs and SATB1- binding sites provide a unique chromatin context which seems preferentially targeted by the HIV-1 integration machinery. Moreover, HIV-1 Tat may overcome SATB1-mediated repression of IL2 and IL2RA (interleukin) in T-cells by binding to the same domain than HDAC1. Delineates specific epigenetic modifications at target gene loci, directly up-regulating metastasis-associated genes while down-regulating tumor-suppressor genes. Reprograms chromatin organization and the transcription profiles of breast tumors to promote growth and metastasis. Interacts (via DNA-binding domains) with CUX1; leading to inhibit the attachment to DNA. Homodimer. Part of the nuclear protein complex gamma-globin promoter and enhancer binding factor (gamma-PE) composed at least by SATB1 and HOXB2. Interaction with CtBP1 when not acetylated stabalizes attachment to DNA and promotes transcription repression. Interacts with PCAF. Interacts with sumoylated PML, HDAC1 and HIV-1 Tat via the PDZ- like dimerization domain. Interacts also with DYNLT3 and POLR2J2. Binds to EP300. Expressed predominantly in thymus. Belongs to the CUT homeobox family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: 3p23 Cellular Component: nucleoplasm; PML body; nuclear matrix; nuclear heterochromatin; nucleus Molecular Function:protein binding; double-stranded DNA binding; chromatin binding Biological Process: establishment and/or maintenance of chromatin architecture; CD8-positive, alpha-beta T cell differentiation; epidermis development; transcription, DNA-dependent; CD4-positive, alpha beta T cell differentiation; viral reproduction; apoptosis; activated T cell proliferation; negative regulation of transcription from RNA polymerase II promoter; histone methylation; chromatin remodeling; reflex; cell structure disassembly during apoptosis
NCBI Summary:	This gene encodes a matrix protein which binds nuclear matrix and scaffold-associating DNAs through a unique nuclear architecture. The protein recruits chromatin-remodeling factors in order to regulate chromatin structure and gene expression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010]
UniProt Code:	Q01826
NCBI GenInfo Identifier:	417747
NCBI Gene ID:	6304
NCBI Accession:	Q01826.1
UniProt Secondary Accession:	Q01826,Q59EQ0, B3KXF1, C9JTR6,
UniProt Related Accession:	Q01826
Molecular Weight:	763
NCBI Full Name:	DNA-binding protein SATB1
NCBI Synonym Full Names:	SATB homeobox 1
NCBI Official Symbol:	SATB1
NCBI Protein Information:	DNA-binding protein SATB1; special AT-rich sequence-binding protein 1; special AT-rich sequence binding protein 1 (binds to nuclear matrix/scaffold-associating DNA)
UniProt Protein Name:	DNA-binding protein SATB1
UniProt Synonym Protein Names:	Special AT-rich sequence-binding protein 1
Protein Family:	DNA-binding protein
UniProt Gene Name:	SATB1
UniProt Entry Name:	SATB1_HUMAN

	Western blot analysis of extracts of Jurkat cells, using SATB1 antibody (CAB5800) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded rat brain using SATB1 antibody (CAB5800) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using SATB1 antibody (CAB5800) at dilution of 1:100 (40x lens).