The SARS-CoV Spike S1 Antibody (CAB20604) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to the SARS-CoV spike S1 protein and has been validated for use in a variety of applications, including Western blotting and immunofluorescence.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against SARS-CoV samples.
Product Name:
SARS-CoV Spike S1 Antibody
SKU:
CAB20604
Size:
20μL, 100μL
Reactivity:
SARS-CoV
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
E2, SARS-CoV-2 Spike S1
Positive Sample:
293T
Calculated MW:
139kDa
Observed MW:
200kDa
The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
Purification Method
Affinity purification
Gene ID
1489668
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SARS-CoV-2 Spike S1 Rabbit pAb (CAB20604) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
