Epigenetics & Nuclear Signaling Antibodies 1

Anti-SAP130 Antibody (CAB13898)

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SKU:
CAB13898
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-SAP130 Antibody
Antibody SKU:CAB13898
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 819-1048 of human SAP130 (NP_078821.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 819-1048 of human SAP130 (NP_078821.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LSMP TSDL PPGA SPRK KPRK QQHV ISTE EGDM METN STDD EKST AKSL LVKA EKRK SPPK EYID EEGV RYVP VRPR PPIT LLRH YRNP WKAA YHHF QRYS DVRV KEEK KAML QEIA NQKG VSCR AQGW KVHL CAAQ LLQL TNLE HDVY ERLT NLQE GIIP KKKA ATDD DLHR INEL IQGN MQRC KLVM DQIS EARD SMLK VLDH KDRV LKLL NKNG TVKK VSKL KRKE KV
Gene ID:79595
Uniprot:Q9H0E3
Cellular Location:Nucleus
Calculated MW:65kDa/110kDa/113kDa
Observed MW:140kDa
Synonyms:SAP130
Background:
UniProt Protein Function:SAP130: Acts as a transcriptional repressor. May function in the assembly and/or enzymatic activity of the mSin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes. Belongs to the SAP130 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transcription factor; Acetyltransferase

Chromosomal Location of Human Ortholog: 2q14.3

Cellular Component: nucleoplasm

Molecular Function:histone acetyltransferase activity; transcription coactivator activity

Biological Process: establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; negative regulation of gene expression, epigenetic; gene expression; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic

NCBI Summary:SAP130 is a subunit of the histone deacetylase (see HDAC1; MIM 601241)-dependent SIN3A (MIM 607776) corepressor complex (Fleischer et al., 2003 [PubMed 12724404]).[supplied by OMIM, Mar 2008]
UniProt Code:Q9H0E3
NCBI GenInfo Identifier:74717977
NCBI Gene ID:79595
NCBI Accession:Q9H0E3.1
UniProt Related Accession:Q9H0E3
Molecular Weight:
NCBI Full Name:Histone deacetylase complex subunit SAP130
NCBI Synonym Full Names:Sin3A associated protein 130
NCBI Official Symbol:SAP130  
NCBI Protein Information:histone deacetylase complex subunit SAP130
UniProt Protein Name:Histone deacetylase complex subunit SAP130
UniProt Synonym Protein Names:130 kDa Sin3-associated polypeptide; Sin3-associated polypeptide p130
UniProt Gene Name:SAP130  
UniProt Entry Name:SP130_HUMAN
Anti-SAP130 Antibody (CAB13898)Western blot analysis of extracts of HeLa cells, using SAP130 antibody (CAB13898) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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