Anti-S100P Antibody (CAB20795)
- SKU:
- CAB20795
- Product Type:
- Antibody
- Antibody Type:
- Secondary Antibody
- Host Species:
- Donkey
- Isotype:
- Alexa Fluor 488 conjugated IgG
Description
| Antibody Name: | Anti-S100P Antibody (CAB20795) |
| Antibody SKU: | CAB20795 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting, Immunofluorescence |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-95 of human S100P (NP_005971.1). |
| Application: | Western blotting, Immunofluorescence |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human |
| Positive Samples: | HT-29 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-95 of human S100P (NP_005971.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MTEL ETAM GMII DVFS RYSG SEGS TQTL TKGE LKVL MEKE LPGF LQSG KDKD AVDK LLKD LDAN GDAQ VDFS EFIV FVAA ITSA CHKY FEKA GLK |
| Cellular Location: | Cell projection, Cytoplasm, Nucleus, microvillus membrane |
| Calculated MW: | 10kDa |
| Observed MW: | 9kDa |
| Synonyms: | MIG9 |
| Background: | The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 4p16. This protein, in addition to binding Ca2+, also binds Zn2+ and Mg2+. This protein may play a role in the etiology of prostate cancer. |
 | Immunofluorescence analysis of human placenta cells using S100P Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Western blot analysis of extracts of HT-29 cells, using S100P antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. |
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