Antibody Name:	Anti-RPS3A Antibody
Antibody SKU:	CAB5885
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human RPS3A (NP_000997.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, 293T, A-549, Mouse liver, Mouse lung, Mouse ovary, Mouse pancreas, Rat ovary

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human RPS3A (NP_000997.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAVG KNKR LTKG GKKG AKKK VVDP FSKK DWYD VKAP AMFN IRNI GKTL VTRT QGTK IASD GLKG RVFE VSLA DLQN DEVA FRKF KLIT EDVQ GKNC LTNF HGMD LTRD KMCS MVKK WQTM IEAH VDVK TTDG YLLR LFCV GFTK KRNN QIRK TSYA QHQQ VRQI RKKM MEIM TREV QTND LKEV VNKL IPDS IGKD IEKA CQSI YPLH DVFV RKVK MLKK PKFE LGKL MELH GEGS SSGK ATGD ETGA KVER ADGY EPPV QESV
Gene ID:	6189
Uniprot:	P61247
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	29kDa
Observed MW:	35kDa

Synonyms:

RPS3A, FTE1, MFTL, S3A

Background:

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S3AE family of ribosomal proteins. It is located in the cytoplasm. Disruption of the gene encoding rat ribosomal protein S3a, also named v-fos transformation effector protein, in v-fos-transformed rat cells results in reversion of the transformed phenotype. This gene is co-transcribed with the U73A and U73B small nucleolar RNA genes, which are located in its fourth and third introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants have been found for this gene.

UniProt Protein Function:	RPS3A: ribosomal protein S3a. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. Belongs to the S3AE family of ribosomal proteins. Located in the cytoplasm. Also named v-fos transformation effector protein.
UniProt Protein Details:	Protein type:Ribosomal; Translation Chromosomal Location of Human Ortholog: 4q31.2-q31.3 Cellular Component: focal adhesion; cytoplasm; nucleolus; nucleus; ribonucleoprotein complex; cytosol Molecular Function:protein binding; structural constituent of ribosome; RNA binding Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translational elongation; viral reproduction; translation; translational initiation; mRNA catabolic process, nonsense-mediated decay; viral transcription; gene expression; translational termination; viral infectious cycle; cell differentiation; negative regulation of apoptosis
NCBI Summary:	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S3AE family of ribosomal proteins. It is located in the cytoplasm. Disruption of the gene encoding rat ribosomal protein S3a, also named v-fos transformation effector protein, in v-fos-transformed rat cells results in reversion of the transformed phenotype. This gene is co-transcribed with the U73A and U73B small nucleolar RNA genes, which are located in its fourth and third introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2012]
UniProt Code:	P61247
NCBI GenInfo Identifier:	47117764
NCBI Gene ID:	6189
NCBI Accession:	P61247.2
UniProt Secondary Accession:	P61247,P33443, P49241, B2R4D4, D3DP05,
UniProt Related Accession:	P61247
Molecular Weight:	264
NCBI Full Name:	40S ribosomal protein S3a
NCBI Synonym Full Names:	ribosomal protein S3A
NCBI Official Symbol:	RPS3A
NCBI Official Synonym Symbols:	S3A; FTE1; MFTL
NCBI Protein Information:	40S ribosomal protein S3a; RPS3A; fte-1; v-fos transformation effector protein 1
UniProt Protein Name:	40S ribosomal protein S3a
UniProt Synonym Protein Names:	v-fos transformation effector protein; Fte-1
Protein Family:	40S ribosomal protein
UniProt Gene Name:	RPS3A
UniProt Entry Name:	RS3A_HUMAN

	Western blot analysis of extracts of various cell lines, using RPS3A antibody (CAB5885) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat brain using RPS3A Antibody (CAB5885) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver cancer using RPS3A Antibody (CAB5885) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using RPS3A Antibody (CAB5885) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of MCF-7 cells using RPS3A antibody (CAB5885). Blue: DAPI for nuclear staining.