Antibody Name:	Anti-RPS27 Antibody
Antibody SKU:	CAB6729
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-84 of human RPS27 (NP_001021.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Rat
Positive Samples:	MCF7, HeLa, H460, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-84 of human RPS27 (NP_001021.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPLA KDLL HPSP EEEK RKHK KKRL VQSP NSYF MDVK CPGC YKIT TVFS HAQT VVLC VGCS TVLC QPTG GKAR LTEG CSFR RKQH
Gene ID:	6232
Uniprot:	P42677
Cellular Location:
Calculated MW:	9kDa
Observed MW:	10kDa

Synonyms:

RPS27, MPS-1, MPS1, S27

Background:

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S27E family of ribosomal proteins. It contains a C4-type zinc finger domain that can bind to zinc. The encoded protein has been shown to be able to bind to nucleic acid. It is located in the cytoplasm as a ribosomal component, but it has also been detected in the nucleus. Studies in rat indicate that ribosomal protein S27 is located near ribosomal protein S18 in the 40S subunit and is covalently linked to translation initiation factor eIF3. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

UniProt Protein Function:	RPS27: a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S27E family of ribosomal proteins. It contains a C4-type zinc finger domain that can bind to zinc. The encoded protein has been shown to be able to bind to nucleic acid. It is located in the cytoplasm as a ribosomal component, but it has also been detected in the nucleus. Studies in rat indicate that ribosomal protein S27 is located near ribosomal protein S18 in the 40S subunit and is covalently linked to translation initiation factor eIF3. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:Translation; Ribosomal Chromosomal Location of Human Ortholog: 1q21 Cellular Component: cytosol; nucleoplasm; nucleus Molecular Function:protein binding; structural constituent of ribosome Biological Process: mRNA catabolic process, nonsense-mediated decay; ribosomal small subunit assembly and maintenance; rRNA processing; sister chromatid cohesion; SRP-dependent cotranslational protein targeting to membrane; translation; translational initiation; viral transcription
NCBI Summary:	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S27E family of ribosomal proteins. It contains a C4-type zinc finger domain that can bind to zinc. The encoded protein has been shown to be able to bind to nucleic acid. It is located in the cytoplasm as a ribosomal component, but it has also been detected in the nucleus. Studies in rat indicate that ribosomal protein S27 is located near ribosomal protein S18 in the 40S subunit and is covalently linked to translation initiation factor eIF3. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Code:	P42677
NCBI GenInfo Identifier:	1171014
NCBI Gene ID:	6232
NCBI Accession:	P42677.3
UniProt Secondary Accession:	P42677,Q5T4L6,
UniProt Related Accession:	P42677
Molecular Weight:	9,461 Da
NCBI Full Name:	40S ribosomal protein S27
NCBI Synonym Full Names:	ribosomal protein S27
NCBI Official Symbol:	RPS27
NCBI Official Synonym Symbols:	S27; MPS1; MPS-1
NCBI Protein Information:	40S ribosomal protein S27
UniProt Protein Name:	40S ribosomal protein S27
UniProt Synonym Protein Names:	Metallopan-stimulin 1; MPS-1
Protein Family:	40S ribosomal protein
UniProt Gene Name:	RPS27
UniProt Entry Name:	RS27_HUMAN

	Western blot analysis of extracts of various cell lines, using RPS27 antibody (CAB6729) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat brain using RPS27 Antibody (CAB6729) at dilution of 1:100 (40x lens).