Antibody Name:	Anti-RPS18 Antibody
Antibody SKU:	CAB11687
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human RPS18 (NP_072045.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, HT-29, LO2, SKOV3, A375, Mouse brain, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human RPS18 (NP_072045.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSLV IPEK FQHI LRVL NTNI DGRR KIAF AITA IKGV GRRY AHVV LRKA DIDL TKRA GELT EDEV ERVI TIMQ NPRQ YKIP DWFL NRQK DVKD GKYS QVLA NGLD NKLR EDLE RLKK IRAH RGLR HFWG LRVR GQHT KTTG RRGR TVGV SKKK
Gene ID:	6222
Uniprot:	P62269
Cellular Location:	Cytoplasm
Calculated MW:	17kDa
Observed MW:	18kDa

Synonyms:

RPS18, D6S218E, HKE3, KE-3, KE3, S18

Background:

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S13P family of ribosomal proteins. It is located in the cytoplasm. The gene product of the E. coli ortholog (ribosomal protein S13) is involved in the binding of fMet-tRNA, and thus, in the initiation of translation. This gene is an ortholog of mouse Ke3. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

UniProt Protein Function:	RPS18: Located at the top of the head of the 40S subunit, it contacts several helices of the 18S rRNA. Belongs to the ribosomal protein S13P family.
UniProt Protein Details:	Protein type:Ribosomal; Translation Chromosomal Location of Human Ortholog: 6p21.32 Cellular Component: cytosol; extracellular matrix; focal adhesion; membrane; nucleoplasm; nucleus; small ribosomal subunit Molecular Function:protein binding; RNA binding Biological Process: mRNA catabolic process, nonsense-mediated decay; ribosome biogenesis and assembly; rRNA processing; SRP-dependent cotranslational protein targeting to membrane; translation; translational initiation; viral transcription
NCBI Summary:	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S13P family of ribosomal proteins. It is located in the cytoplasm. The gene product of the E. coli ortholog (ribosomal protein S13) is involved in the binding of fMet-tRNA, and thus, in the initiation of translation. This gene is an ortholog of mouse Ke3. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Code:	P62269
NCBI GenInfo Identifier:	50403625
NCBI Gene ID:	6222
NCBI Accession:	P62269.3
UniProt Secondary Accession:	P62269,P25232, Q5SUJ3, Q6IPF8,
UniProt Related Accession:	P62269
Molecular Weight:	18kDa
NCBI Full Name:	40S ribosomal protein S18
NCBI Synonym Full Names:	ribosomal protein S18
NCBI Official Symbol:	RPS18
NCBI Official Synonym Symbols:	KE3; S18; HKE3; KE-3; D6S218E
NCBI Protein Information:	40S ribosomal protein S18
UniProt Protein Name:	40S ribosomal protein S18
UniProt Synonym Protein Names:	Ke-3; Ke3; Small ribosomal subunit protein uS13
Protein Family:	40S ribosomal protein
UniProt Gene Name:	RPS18

	Western blot analysis of extracts of various cell lines, using RPS18 antibody (CAB11687) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded Human thyroid cancer using RPS18 antibody (CAB11687) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Mouse kidney using RPS18 antibody (CAB11687) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Rat brain using RPS18 antibody (CAB11687) at dilution of 1:100 (40x lens).