Cell Biology Antibodies 3
Anti-RPL32 Antibody (CAB13001)
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- SKU:
- CAB13001
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-RPL32 Antibody |
| Antibody SKU: | CAB13001 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human RPL32 (NP_000985.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, HepG2, NIH/3T3, Mouse liver, Mouse stomach, Mouse spleen, Rat ovary |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human RPL32 (NP_000985.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAL RPLV KPKI VKKR TKKF IRHQ SDRY VKIK RNWR KPRG IDNR VRRR FKGQ ILMP NIGY GSNK KTKH MLPS GFRK FLVH NVKE LEVL LMCN KSYC AEIA HNVS SKNR KAIV ERAA QLAI RVTN PNAR LRSE ENE |
| Gene ID: | 6161 |
| Uniprot: | P62910 |
| Cellular Location: | |
| Calculated MW: | 15kDa |
| Observed MW: | 16kDa |
| Synonyms: | RPL32, L32, PP9932 |
| Background: | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L32E family of ribosomal proteins. It is located in the cytoplasm. Although some studies have mapped this gene to 3q13.3-q21, it is believed to map to 3p25-p24. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants encoding the same protein have been observed for this gene. |
| NCBI Summary: | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L32E family of ribosomal proteins. It is located in the cytoplasm. Although some studies have mapped this gene to 3q13.3-q21, it is believed to map to 3p25-p24. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants encoding the same protein have been observed for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P62910 |
| NCBI GenInfo Identifier: | 4506635 |
| NCBI Gene ID: | 6161 |
| NCBI Accession: | NP_000985 |
| UniProt Related Accession: | P62910 |
| Molecular Weight: | 15kDa |
| NCBI Full Name: | 60S ribosomal protein L32 |
| NCBI Synonym Full Names: | ribosomal protein L32 |
| NCBI Official Symbol: | RPL32 |
| NCBI Official Synonym Symbols: | L32; PP9932 |
| NCBI Protein Information: | 60S ribosomal protein L32 |
| UniProt Protein Name: | 60S ribosomal protein L32 |
| Protein Family: | 50S ribosomal protein |
| UniProt Gene Name: | RPL32 |
| UniProt Entry Name: | RL32_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RPL32 antibody (CAB13001) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded rat brain using RPL32 antibody (CAB13001) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human lung cancer using RPL32 antibody (CAB13001) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse spinal cord using RPL32 antibody (CAB13001) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of L929 cells using RPL32 Polyclonal Antibody (CAB13001) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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