Cell Biology Antibodies 9
Anti-RPL13 Antibody (CAB4083)
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- SKU:
- CAB4083
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-RPL13 Antibody |
| Antibody SKU: | CAB4083 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human RPL13 (NP_150254.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, HT-29, BxPC-3, 293T, HeLa, Mouse spleen, Mouse thymus, Mouse lung, Rat lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human RPL13 (NP_150254.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAPS RNGM VLKP HFHK DWQR RVAT WFNQ PARK IRRR KARQ AKAR RIAP RPAS GPIR PIVR CPTV RYHT KVRA GRGF SLEE LRVA GIHK KVAR TIGI SVDP RRRN KSTE SLQA NVQR LKEY RSKL ILFP RKPS APKK GDSS AEEL KLAT QLTG PVMP VRNV YKKE KARV ITEE EKNF KAFA SLRM ARAN ARLF GIRA KRAK EAAE QDVE KKK |
| Gene ID: | 6137 |
| Uniprot: | P26373 |
| Cellular Location: | |
| Calculated MW: | 18kDa/24kDa |
| Observed MW: | 28kDa |
| Synonyms: | RPL13, BBC1, D16S444E, D16S44E, L13 |
| Background: | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L13E family of ribosomal proteins. It is located in the cytoplasm. This gene is expressed at significantly higher levels in benign breast lesions than in breast carcinomas. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. |
| UniProt Protein Function: | RPL13: a ribosomal protein. A component of the 60S ribosomal subunit. Reduced expression observed in breast cancer. |
| UniProt Protein Details: | Protein type:Translation; Ribosomal Chromosomal Location of Human Ortholog: 16q24.3|17p11.2 Cellular Component: cytosol; membrane; nucleus Molecular Function:protein binding; RNA binding Biological Process: mRNA catabolic process, nonsense-mediated decay; rRNA processing; SRP-dependent cotranslational protein targeting to membrane; translational initiation; viral transcription |
| NCBI Summary: | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L13E family of ribosomal proteins. It is located in the cytoplasm. This gene is expressed at significantly higher levels in benign breast lesions than in breast carcinomas. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2011] |
| UniProt Code: | P26373 |
| NCBI GenInfo Identifier: | 21903462 |
| NCBI Gene ID: | 6137 |
| NCBI Accession: | P26373.4 |
| UniProt Secondary Accession: | P26373,Q3KQT8, Q567Q8, Q9BPX0, B4DLX3, F5H1S2, |
| UniProt Related Accession: | P26373 |
| Molecular Weight: | 18,845 Da |
| NCBI Full Name: | 60S ribosomal protein L13 |
| NCBI Synonym Full Names: | ribosomal protein L13 |
| NCBI Official Symbol: | RPL13 |
| NCBI Official Synonym Symbols: | L13; BBC1; D16S44E; D16S444E |
| NCBI Protein Information: | 60S ribosomal protein L13 |
| UniProt Protein Name: | 60S ribosomal protein L13 |
| UniProt Synonym Protein Names: | Breast basic conserved protein 1 |
| Protein Family: | 60S ribosomal protein |
| UniProt Gene Name: | RPL13 |
| UniProt Entry Name: | RL13_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RPL13 antibody (CAB4083) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
 | Immunohistochemistry of paraffin-embedded rat brain using RPL13 antibody (CAB4083) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat spleen using RPL13 antibody (CAB4083) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human lung using RPL13 antibody (CAB4083) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of MCF7 cells using RPL13 antibody (CAB4083). Blue: DAPI for nuclear staining. |
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