Epigenetics & Nuclear Signaling Antibodies 5

RPA70/RPA1 Rabbit Monoclonal Antibody (CAB3367)

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SKU:
CAB3367
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling
Antibody Type:
Monoclonal Antibody
Reactivity:
Human, Rat
Synonyms:
HSSB, RF-A, RP-A, REPA1, RPA70, MST075, PFBMFT6, RPA70/RPA1
Research Use:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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RPA70/RPA1 Monoclonal Antibody (CAB3367)

The RPA70/RPA1 Monoclonal Antibody (CAB3367) is a high-quality antibody developed for reliable detection and analysis of target proteins. RPA70, a subunit of the replication protein A complex, plays a crucial role in DNA replication, repair, and recombination.This antibody, generated in rabbits, shows high specificity and sensitivity in detecting RPA70 protein in various cell types and tissues. It is suitable for use in Western blot, immunohistochemistry, and immunofluorescence applications, allowing for detailed analysis and visualization of RPA70 localization and expression levels.

This antibody is validated for use in WB, IP, ChIP, ELISA applications and has demonstrated reactivity against Human, Rat samples.

Product Name:RPA70/RPA1 Monoclonal Antibody
SKU:CAB3367
Size:20μL, 100μL
Reactivity:Human, Rat
Clone Number:ARC0773
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:MVGQ LSEG AIAA IMQK GDTN IKPI LQVI NIRP ITTG NSPP RYRL LMSD GLNT LSSF MLAT QLNP LVEE EQLS SNCV CQIH RFIV NTLK DGRR VVIL MELE
Tested Applications:WB IP ChIP ELISA
Recommended Dilution:
WB1:500 - 1:2000
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
ChIP5μg antibody for 10μg-15μg of Chromatin
Synonyms:HSSB, RF-A, RP-A, REPA1, RPA70, MST075, PFBMFT6, RPA70/RPA1
Positive Sample:HeLa, U-251 MG
Cellular Localization:Nucleus, Pml Body.
Calculated MW:68kDa
Observed MW:70kDa

This gene encodes the largest subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The nucleoprotein complex protects the single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. This subunit contains four oligonucleotide/oligosaccharide-binding (OB) domains, though the majority of ssDNA binding occurs in two of these domains. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which ssDNA binding domains are utilized. The different binding modes differ in the length of DNA bound and in the proteins with which it interacts, thereby playing a role in regulating different genomic maintenance pathways.

Purification MethodAffinity purification
Gene ID6117
RRIDAB_2863044
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using RPA70/RPA1 Rabbit mAb (CAB3367) at 1：1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageImmunoprecipitation of RPA70/RPA1 from 300 µg extracts of HeLa cells was performed using 2 µg of RPA70/RPA1 Rabbit mAb (CAB3367). Rabbit IgG isotype control (AC005) was used to precipitate the Control IgG sample. IP samples were eluted with 1X reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using RPA70/RPA1 Rabbit mAb (CAB3367) at a dilution of 1:1000.
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