Name: Anti-RORA Antibody (CAB6971)
Brand: Cell Biology Antibodies 10
SKU: CAB6971
Availability: OutOfStock

Antibody Name:	Anti-RORA Antibody
Antibody SKU:	CAB6971
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 169-468 of human RORA (NP_599024.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse brain, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 169-468 of human RORA (NP_599024.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DIQP SPDQ SGLD INGI KPEP ICDY TPAS GFFP YCSF TNGE TSPT VSMA ELEH LAQN ISKS HLET CQYL REEL QQIT WQTF LQEE IENY QNKQ REVM WQLC AIKI TEAI QYVV EFAK RIDG FMEL CQND QIVL LKAG SLEV VFIR MCRA FDSQ NNTV YFDG KYAS PDVF KSLG CEDF ISFV FEFG KSLC SMHL TEDE IALF SAFV LMSA DRSW LQEK VKIE KLQQ KIQL ALQH VLQK NHRE DGIL TKLI CKVS TLRA LCGR HTEK LMAF KAIY PDIV RLHF PPLY KELF TSEF EPAM QIDG
Gene ID:	6095
Uniprot:	P35398
Cellular Location:	Nucleus
Calculated MW:	53kDa/58kDa/61kDa/63kDa
Observed MW:	63kDa

Synonyms:

RORA, NR1F1, ROR1, ROR2, ROR3, RZR-ALPHA, RZRA

Background:

The protein encoded by this gene is a member of the NR1 subfamily of nuclear hormone receptors. It can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The encoded protein has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, as well as with NM23-1, the product of a tumor metastasis suppressor candidate gene. Also, it has been shown to aid in the transcriptional regulation of some genes involved in circadian rhythm. Four transcript variants encoding different isoforms have been described for this gene.

UniProt Protein Function:	Nuclear receptor that binds DNA as a monomer to ROR response elements (RORE) containing a single core motif half-site 5'-AGGTCA-3' preceded by a short A-T-rich sequence. Key regulator of embryonic development, cellular differentiation, immunity, circadian rhythm as well as lipid, steroid, xenobiotics and glucose metabolism. Considered to have intrinsic transcriptional activity, have some natural ligands like oxysterols that act as agonists (25-hydroxycholesterol) or inverse agonists (7-oxygenated sterols), enhancing or repressing the transcriptional activity, respectively. Recruits distinct combinations of cofactors to target genes regulatory regions to modulate their transcriptional expression, depending on the tissue, time and promoter contexts. Regulates genes involved in photoreceptor development including OPN1SW, OPN1SM and ARR3 and skeletal muscle development with MYOD1. Required for proper cerebellum development, regulates SHH gene expression, among others, to induce granule cells proliferation as well as expression of genes involved in calcium-mediated signal transduction. Regulates the circadian expression of several clock genes, including CLOCK, ARNTL/BMAL1, NPAS2 and CRY1. Competes with NR1D1 for binding to their shared DNA response element on some clock genes such as ARNTL/BMAL1, CRY1 and NR1D1 itself, resulting in NR1D1-mediated repression or RORA-mediated activation of clock genes expression, leading to the circadian pattern of clock genes expression. Therefore influences the period length and stability of the clock. Regulates genes involved in lipid metabolism such as apolipoproteins APOA1, APOA5, APOC3 and PPARG. In liver, has specific and redundant functions with RORC as positive or negative modulator of expression of genes encoding phase I and phase II proteins involved in the metabolism of lipids, steroids and xenobiotics, such as CYP7B1 and SULT2A1. Induces a rhythmic expression of some of these genes. In addition, interplays functionally with NR1H2 and NR1H3 for the regulation of genes involved in cholesterol metabolism. Also involved in the regulation of hepatic glucose metabolism through the modulation of G6PC and PCK1. In adipose tissue, plays a role as negative regulator of adipocyte differentiation, probably acting through dual mechanisms. May suppress CEBPB-dependent adipogenesis through direct interaction and PPARG-dependent adipogenesis through competition for DNA-binding. Downstream of IL6 and TGFB and synergistically with RORC isoform 2, is implicated in the lineage specification of uncommitted CD4+ T-helper (T(H)) cells into T(H)17 cells, antagonizing the T(H)1 program. Probably regulates IL17 and IL17F expression on T(H) by binding to the essential enhancer conserved non-coding sequence 2 (CNS2) in the IL17-IL17F locus. Involved in hypoxia signaling by interacting with and activating the transcriptional activity of HIF1A. May inhibit cell growth in response to cellular stress. May exert an anti-inflammatory role by inducing CHUK expression and inhibiting NF-kappa-B signaling.
NCBI Summary:	The protein encoded by this gene is a member of the NR1 subfamily of nuclear hormone receptors. It can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The encoded protein has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, as well as with NM23-1, the product of a tumor metastasis suppressor candidate gene. Also, it has been shown to aid in the transcriptional regulation of some genes involved in circadian rhythm. Four transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2014]
UniProt Code:	P35398
NCBI GenInfo Identifier:	613504304
NCBI Gene ID:	6095
NCBI Accession:	P35398.2
UniProt Secondary Accession:	P35398,P35397, P35399, P45445, Q495X4, Q96H83,
UniProt Related Accession:	P35398
Molecular Weight:
NCBI Full Name:	Nuclear receptor ROR-alpha
NCBI Synonym Full Names:	RAR related orphan receptor A
NCBI Official Symbol:	RORA
NCBI Official Synonym Symbols:	ROR1; ROR2; ROR3; RZRA; NR1F1; RZR-ALPHA
NCBI Protein Information:	nuclear receptor ROR-alpha
UniProt Protein Name:	Nuclear receptor ROR-alpha
UniProt Synonym Protein Names:	Nuclear receptor RZR-alpha; Nuclear receptor subfamily 1 group F member 1; RAR-related orphan receptor A; Retinoid-related orphan receptor-alpha
Protein Family:	Nuclear receptor
UniProt Gene Name:	RORA
UniProt Entry Name:	RORA_HUMAN

Western blot analysis of extracts of various cell lines, using RORA antibody (CAB6971) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

RORA Antibody (PACO01459)

RORA Antibody (PACO11890)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)