KO Validated Antibodies 1

Anti-ROCK1 Antibody (CAB1008)[KO Validated]

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SKU:
CAB1008

Description

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Antibody Name:Anti-ROCK1 Antibody [KO Validated]
Antibody SKU:CAB1008
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human ROCK1 (NP_005397.1).
Application:WB IHC IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IP 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:293T, U-251MG, Jurkat, BT-474
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human ROCK1 (NP_005397.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:YSNR RYLS SANP NDNR TSSN ADKS LQES LQKT IYKL EEQL HNEM QLKD EMEQ KCRT SNIK LDKI MKEL DEEG NQRR NLES TVSQ IEKE KMLL QHRI NEYQ RKAE QENE KRRN VENE VSTL KDQL EDLK KVSQ NSQL ANEK LSQL QKQL EEAN DLLR TESD TAVR LRKS HTEM SKSI SQLE SLNR ELQE RNRI LENS KSQT DKDY YQLQ AILE AERR DRGH DSEM IGDL QARI TSLQ EEVK HLKH NLEK VEGE RKEA QDML N
Gene ID:6093
Uniprot:Q13464
Cellular Location:Cell membrane, Cell projection, Cytoplasm, Golgi apparatus membrane, Peripheral membrane protein, bleb, centriole, centrosome, cytoskeleton, lamellipodium, microtubule organizing center, ruffle
Calculated MW:158kDa
Observed MW:158kDa
Synonyms:ROCK1, P160ROCK, ROCK-I
Background:This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18.
UniProt Protein Function:Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization.
NCBI Summary:This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18. [provided by RefSeq, Aug 2015]
UniProt Code:Q13464
NCBI GenInfo Identifier:47605999
NCBI Gene ID:6093
NCBI Accession:Q13464.1
UniProt Secondary Accession:Q13464,Q2KHM4, Q59GZ4, B0YJ91,
UniProt Related Accession:Q13464
Molecular Weight:185 kDa
NCBI Full Name:Rho-associated protein kinase 1
NCBI Synonym Full Names:Rho associated coiled-coil containing protein kinase 1
NCBI Official Symbol:ROCK1  
NCBI Official Synonym Symbols:ROCK-I; P160ROCK  
NCBI Protein Information:rho-associated protein kinase 1
UniProt Protein Name:Rho-associated protein kinase 1
UniProt Synonym Protein Names:Renal carcinoma antigen NY-REN-35; Rho-associated, coiled-coil-containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase I; ROCK-I; p160 ROCK-1; p160ROCK
Protein Family:Rho-associated protein kinase
UniProt Gene Name:ROCK1  
Anti-ROCK1 Antibody [KO Validated] (CAB1008)Western blot analysis of extracts of various cell lines, using ROCK1 antibody (CAB1008) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-ROCK1 Antibody [KO Validated] (CAB1008)Western blot analysis of extracts from normal (control) and ROCK1 knockout (KO) HeLa cells, using ROCK1 antibody (CAB1008) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
Anti-ROCK1 Antibody [KO Validated] (CAB1008)Immunohistochemistry of paraffin-embedded mouse spleen using ROCK1 antibody (CAB1008) at dilution of 1:100 (40x lens).
Anti-ROCK1 Antibody [KO Validated] (CAB1008)Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug ROCK1 antibody (CAB1008). Western blot was performed from the immunoprecipitate using ROCK1 antibody (CAB1008) at a dilition of 1:1000.
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Additional Information

Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
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