Immunology Antibodies 2
Anti-RNGTT Antibody (CAB6431)
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- SKU:
- CAB6431
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-RNGTT Antibody |
| Antibody SKU: | CAB6431 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human RNGTT (NP_003791.3). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, THP-1, 293T, Jurkat, HeLa, Mouse brain, Mouse spleen, Mouse kidney, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human RNGTT (NP_003791.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAHN KIPP RWLN CPRR GQPV AGRF LPLK TMLG PRYD SQVA EENR FHPS MLSN YLKS LKVK MGLL VDLT NTSR FYDR NDIE KEGI KYIK LQCK GHGE CPTT ENTE TFIR LCER FNER NPPE LIGV HCTH GFNR TGFL ICAF LVEK MDWS IEAA VATF AQAR PPGI YKGD YLKE LFRR YGDI EEAP PPPL LPDW CFED DEDE DEDE DGKK ESEP GSSA SFGK RRKE RLKL GAIF LEGV TVKG VTQV TTQP KLGE VQQK CHQF |
| Gene ID: | 8732 |
| Uniprot: | O60942 |
| Cellular Location: | Nucleus |
| Calculated MW: | 52kDa/61kDa/66kDa/68kDa |
| Observed MW: | 69kDa |
| Synonyms: | RNGTT, CAP1A, HCE, HCE1, hCAP |
| Background: |
| UniProt Protein Function: | RNGTT: a bifunctional mRNA capping enzyme with RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus. Four alternatively spliced human isoforms have been described. Isoform 1 and isoform 4 are expressed in cerebrum, cerebellum, thyroid, lung, heart, liver, kidney, spleen, large intestine, testis, skin and muscle. Isoforms 2, 3 and 4 lack mRNA 5'-guanylyltransferase activity due to disruptions of the GTPase domain. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Transferase; Phosphatase (non-protein); EC 2.7.7.50; EC 3.1.3.33 Chromosomal Location of Human Ortholog: 6q16 Cellular Component: nucleoplasm; nucleus Molecular Function:polynucleotide 5'-phosphatase activity; GTP binding; mRNA guanylyltransferase activity; RNA guanylyltransferase activity; protein tyrosine/serine/threonine phosphatase activity; triphosphatase activity; protein tyrosine phosphatase activity Biological Process: transcription from RNA polymerase II promoter; RNA processing; mRNA capping; viral reproduction; gene expression |
| UniProt Code: | O60942 |
| NCBI GenInfo Identifier: | 6685628 |
| NCBI Gene ID: | 8732 |
| NCBI Accession: | O60942.1 |
| UniProt Secondary Accession: | O60942,O43483, O60257, O60351, Q5TCW8, Q8WUM8, E1P513 E1P514, |
| UniProt Related Accession: | O60942 |
| Molecular Weight: | 597 |
| NCBI Full Name: | mRNA-capping enzyme |
| NCBI Synonym Full Names: | RNA guanylyltransferase and 5'-phosphatase |
| NCBI Official Symbol: | RNGTT |
| NCBI Official Synonym Symbols: | HCE; HCE1; hCAP; CAP1A |
| NCBI Protein Information: | mRNA-capping enzyme; HCAP1 |
| UniProt Protein Name: | mRNA-capping enzyme |
| UniProt Synonym Protein Names: | HCAP1; HCEIncluding the following 2 domains:Polynucleotide 5'-triphosphatase (EC:3.1.3.33)Alternative name(s):mRNA 5'-triphosphatase; TPase |
| UniProt Gene Name: | RNGTT |
| UniProt Entry Name: | MCE1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RNGTT antibody (CAB6431) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of MCF7 cells using RNGTT antibody (CAB6431). Blue: DAPI for nuclear staining. |
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