Name: Anti-RNF6 Antibody (CAB14572)
Brand: Signal Transduction Antibodies 1
SKU: CAB14572
Availability: OutOfStock

Antibody Name:	Anti-RNF6 Antibody
Antibody SKU:	CAB14572
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human RNF6 (NP_898864.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	DU145, U-87MG, Mouse testis, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human RNF6 (NP_898864.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MNQS RSRS DGGS EETL PQDH NHHE NERR WQQE RLHR EEAY YQFI NELN DEDY RLMR DHNL LGTP GEIT SEEL QQRL DGVK EQLA SQPD LRDG TNYR DSEV PRES SHED SLLE WLNT FRRT GNAT RSGQ NGNQ TWRA VSRT NPNN GEFR FSLE IHVN HENR GFEI HGED YTDI PLSD SNRD HTAN RQQR STSP VARR TRSQ TSVN FNGS SSNI PRTR LASR GQNP AEGS FSTL GRLR NGIG GAAG IPRA NASR TNFS SHTN QSGG SELR QREG QRFG AAHV WENG ARSN VTVR NTNQ RLEP
Gene ID:	6049
Uniprot:	Q9Y252
Cellular Location:	Cell projection, Cytoplasm, Nucleus, PML body, axon
Calculated MW:	16kDa/37kDa/78kDa
Observed MW:	78kDa

Synonyms:

RNF6

Background:

The protein encoded by this gene contains a RING-H2 finger motif. Deletions and mutations in this gene were detected in esophageal squamous cell carcinoma (ESCC), suggesting that this protein may be a potential tumor suppressor. Studies of the mouse counterpart suggested a role of this protein in the transcription regulation that controls germinal differentiation. Multiple alternatively spliced transcript variants encoding the same protein are observed.

UniProt Protein Function:	RNF6: E3 ubiquitin-protein ligase mediating 'Lys-48'-linked polyubiquitination of LIMK1 and its subsequent targeting to the proteasome for degradation. Negatively regulates axonal outgrowth through regulation of the LIMK1 turnover. Mediates 'Lys-6' and 'Lys-27'-linked polyubiquitination of AR/androgen receptor thereby modulating its transcriptional activity. May also bind DNA and function as a transcriptional regulator. Defects in RNF6 may be a cause of esophageal cancer (ESCR). A malignancy of the esophagus. The most common types are esophageal squamous cell carcinoma and adenocarcinoma. Cancer of the esophagus remains a devastating disease because it is usually not detected until it has progressed to an advanced incurable stage. Belongs to the RNF12 family.
UniProt Protein Details:	Protein type:Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.-; Ligase Chromosomal Location of Human Ortholog: 13q12.2 Cellular Component: axon; cytoplasm; intracellular membrane-bound organelle; nuclear membrane; nucleoplasm; nucleus Molecular Function:androgen receptor binding; protein binding; ubiquitin-protein ligase activity Biological Process: negative regulation of axon extension; positive regulation of transcription, DNA-dependent; proteasomal ubiquitin-dependent protein catabolic process; regulation of transcription, DNA-dependent; ubiquitin-dependent protein catabolic process Disease: Esophageal Cancer
NCBI Summary:	The protein encoded by this gene contains a RING-H2 finger motif. Deletions and mutations in this gene were detected in esophageal squamous cell carcinoma (ESCC), suggesting that this protein may be a potential tumor suppressor. Studies of the mouse counterpart suggested a role of this protein in the transcription regulation that controls germinal differentiation. Multiple alternatively spliced transcript variants encoding the same protein are observed. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9Y252
NCBI GenInfo Identifier:	13124536
NCBI Gene ID:	6049
NCBI Accession:	Q9Y252.1
UniProt Secondary Accession:	Q9Y252,Q5W0H0, Q9BZP5, Q9UF41, B4DDP0,
UniProt Related Accession:	Q9Y252
Molecular Weight:	16,680 Da
NCBI Full Name:	E3 ubiquitin-protein ligase RNF6
NCBI Synonym Full Names:	ring finger protein 6
NCBI Official Symbol:	RNF6
NCBI Protein Information:	E3 ubiquitin-protein ligase RNF6
UniProt Protein Name:	E3 ubiquitin-protein ligase RNF6
Protein Family:	E3 ubiquitin-protein ligase
UniProt Gene Name:	RNF6
UniProt Entry Name:	RNF6_HUMAN

Western blot analysis of extracts of various cell lines, using RNF6 antibody (CAB14572) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

RNF6 Antibody (PACO11876)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)