Name: Anti-RNF34 Antibody (CAB8517)
Brand: Cell Death Antibodies 2
SKU: CAB8517
Availability: OutOfStock

Antibody Name:	Anti-RNF34 Antibody
Antibody SKU:	CAB8517
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human RNF34 (NP_079402.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	U-251MG, LO2, HeLa, BT-474

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human RNF34 (NP_079402.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MKAG ATSM WASC CGLL NEVM GTGA VRGQ QSAF AGAT GPFR FTPN PEFS TYPP AATE GPNI VCKA CGLS FSVF RKKH VCCD CKKD FCSV CSVL QENL RRCS TCHL LQET AFQR PQLM RLKV KDLR QYLI LRNI PIDT CREK EDLV DLVL CHHG LGSE DDMD TSSL NSSR SQTS SFFT RSFF SNYT APSA TMSS FQGE LMDG DQTS RSGV PAQV QSEI TSAN
Gene ID:	80196
Uniprot:	Q969K3
Cellular Location:	Cell membrane, Cytoplasm, Endomembrane system, Nucleus, Nucleus speckle, Peripheral membrane protein, cytosol
Calculated MW:	41kDa
Observed MW:	42kDa

Synonyms:

RNF34, CARP-1, CARP1, RFI, RIF, RIFF, hRFI

Background:

The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. This protein also targets p53 and phospho-p53 for degradation. Alternatively splicing results in multiple transcript variants encoding distinct isoforms.

UniProt Protein Function:	RNF34: Has E3 ubiquitin-protein ligase activity. Regulates the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. Protects cells against apoptosis induced by TNF. Binds phosphatidylinositol 5-phosphate and phosphatidylinositol 3- phosphate. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Ubiquitin conjugating system; Ligase; Ubiquitin ligase; EC 6.3.2.- Chromosomal Location of Human Ortholog: 12q24.31 Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus; plasma membrane Molecular Function:p53 binding; protein binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; protein ubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process
NCBI Summary:	The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. This protein also targets p53 and phospho-p53 for degradation. Alternatively splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2012]
UniProt Code:	Q969K3
NCBI GenInfo Identifier:	74760679
NCBI Gene ID:	80196
NCBI Accession:	Q969K3.1
UniProt Secondary Accession:	Q969K3,Q8NG47, Q9H6W8, B7Z933,
UniProt Related Accession:	Q969K3
Molecular Weight:	41,797 Da
NCBI Full Name:	E3 ubiquitin-protein ligase RNF34
NCBI Synonym Full Names:	ring finger protein 34
NCBI Official Symbol:	RNF34
NCBI Official Synonym Symbols:	RFI; RIF; RIFF; hRFI; CARP1; CARP-1
NCBI Protein Information:	E3 ubiquitin-protein ligase RNF34
UniProt Protein Name:	E3 ubiquitin-protein ligase RNF34
UniProt Synonym Protein Names:	Caspase regulator CARP1
UniProt Gene Name:	RNF34
UniProt Entry Name:	RNF34_HUMAN

Anti-RNF34 Polyclonal Antibody (CAB8517)

Western blot analysis of extracts of various cell lines, using RNF34 antibody (CAB8517) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

RNF34 Antibody (PACO11874)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)