Name: Anti-RNF31 Antibody (CAB8227)
Brand: Signal Transduction Antibodies 3
SKU: CAB8227
Availability: OutOfStock

Antibody Name:	RNF31 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8227
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 500-700 of human RNF31 (NP_060469.4).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Rat
Positive Samples:	Jurkat, MCF7, BT-474, HeLa, PC-3

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 500-700 of human RNF31 (NP_060469.4).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AAGA CPEE IFSA LQYS GTEV PLQW LRSE LPYV LEMV AELA GQQD PGLG AFSC QEAR RAWL DRHG NLDE AVEE CVRT RRRK VQEL QSLG FGPE EGSL QALF QHGG DVSR ALTE LQRQ RLEP FRQR LWDS GPEP TPSW DGPD KQSL VRRL LAVY ALPS WGRA ELAL SLLQ ETPR NYEL GDVV EAVR HSQD RAFL RRLL AQEC A
Gene ID:	55072
Uniprot:	Q96EP0
Cellular Location:	Cytoplasm
Calculated MW:	57kDa/102kDa/119kDa
Observed MW:	125kDa

Synonyms:	RNF31, HOIP, Paul, ZIBRA
Background:	The protein encoded by this gene contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions. The encoded protein is the E3 ubiquitin-protein ligase component of the linear ubiquitin chain assembly complex. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	RNF31: E3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear ('M-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF- induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Binds polyubiquitin of different linkage types. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Ubiquitin ligase; Ligase; Ubiquitin conjugating system; EC 6.3.2.-; EC 6.3.2.19 Chromosomal Location of Human Ortholog: 14q11.2 Cellular Component: cytosol; internal side of plasma membrane Molecular Function:protein binding; ubiquitin binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity Biological Process: activation of NF-kappaB transcription factor; I-kappaB kinase/NF-kappaB cascade; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein polyubiquitination; T cell receptor signaling pathway
NCBI Summary:	The protein encoded by this gene contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions. The encoded protein is the E3 ubiquitin-protein ligase component of the linear ubiquitin chain assembly complex. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2015]
UniProt Code:	Q96EP0
NCBI GenInfo Identifier:	45477216
NCBI Gene ID:	55072
NCBI Accession:	Q96EP0.1
UniProt Secondary Accession:	Q96EP0,Q86VI2, Q8TEI0, Q96GB4, Q96NF1, Q9H5F1, Q9NWD2 A0A962,
UniProt Related Accession:	Q96EP0
Molecular Weight:	102,496 Da
NCBI Full Name:	E3 ubiquitin-protein ligase RNF31
NCBI Synonym Full Names:	ring finger protein 31
NCBI Official Symbol:	RNF31
NCBI Official Synonym Symbols:	HOIP; ZIBRA
NCBI Protein Information:	E3 ubiquitin-protein ligase RNF31
UniProt Protein Name:	E3 ubiquitin-protein ligase RNF31
UniProt Synonym Protein Names:	HOIL-1-interacting protein; HOIP; RING finger protein 31; Zinc in-between-RING-finger ubiquitin-associated domain protein
Protein Family:	E3 ubiquitin-protein ligase
UniProt Gene Name:	RNF31
UniProt Entry Name:	RNF31_HUMAN

Anti-RNF31 Polyclonal Antibody (CAB8227)

Western blot analysis of extracts of various cell lines, using RNF31 antibody (CAB8227) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

Anti-RNF31 Antibody (CAB14139)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)