Developmental Biology
Anti-RNF17 Antibody (CAB13813)
- SKU:
- CAB13813
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Description
| Antibody Name: | Anti-RNF17 Antibody |
| Antibody SKU: | CAB13813 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 900-1200 of human RNF17 (NP_112567.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | A-549 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 900-1200 of human RNF17 (NP_112567.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VSAK SLPN ENFQ SLYN KELP VHIC NVIS PEKI YVQW LLTE NLLN SLEE KMIA AYEN SKWE PVKW ENDM HCAV KIQD KNQW RRGQ IIRM VTDT LVEV LLYD VGVE LVVN VDCL RKLE ENLK TMGR LSLE CSLV DIRP AGGS DKWT ATAC DCLS LYLT GAVA TIIL QVDS EENN TTWP LPVK IFCR DEKG ERVD VSKY LIKK GLAL RERR INNL DNSH SLSE KSLE VPLE QEDS VVTN CIKT NFDP DKKT ADII SEQK VSEF QEKI LEPR TTRG YKPP AIPN MNVF EATV SCVG DDGT IFVV P |
| Gene ID: | 56163 |
| Uniprot: | Q9BXT8 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 50kDa/73kDa/179kDa/183kDa/184kDa |
| Observed MW: | 185kDa |
| Synonyms: | RNF17, Mmip-2, SPATA23, TDRD4 |
| Background: | This gene is similar to a mouse gene that encodes a testis-specific protein containing a RING finger domain. Alternatively spliced transcript variants encoding different isoforms have been found. |
| UniProt Protein Function: | RNF17: Seems to be involved in regulation of transcriptional activity of MYC. In vitro, inhibits DNA-binding activity of Mad- MAX heterodimers. Can recruit Mad transcriptional repressors (MXD1, MXD3, MXD4 and MXI1) to the cytoplasm. May be involved in spermiogenesis. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cancer Testis Antigen (CTA) Chromosomal Location of Human Ortholog: 13q12.12 Cellular Component: cytoplasm; nucleus Molecular Function:protein homodimerization activity; zinc ion binding Biological Process: multicellular organismal development; spermatid development |
| UniProt Code: | Q9BXT8 |
| NCBI GenInfo Identifier: | 94421471 |
| NCBI Gene ID: | |
| NCBI Accession: | NP_112567 |
| Molecular Weight: | |
| NCBI Full Name: | RING finger protein 17 isoform 1 |
| UniProt Protein Name: | RING finger protein 17 |
| UniProt Synonym Protein Names: | Tudor domain-containing protein 4 |
| Protein Family: | RING finger protein |
| UniProt Gene Name: | RNF17 |
| UniProt Entry Name: | RNF17_HUMAN |
 | Western blot analysis of extracts of A-549 cells, using RNF17 antibody (CAB13813) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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