Antibody Name:	Anti-RNASEH2A Antibody
Antibody SKU:	CAB15132
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-299 of human RNASEH2A (NP_006388.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Rat
Positive Samples:	rat ovary, rat testis, rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-299 of human RNASEH2A (NP_006388.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDLS ELER DNTG RCRL SSPV PAVC RKEP CVLG VDEA GRGP VLGP MVYA ICYC PLPR LADL EALK VADS KTLL ESER ERLF AKME DTDF VGWA LDVL SPNL ISTS MLGR VKYN LNSL SHDT ATGL IQYA LDQG VNVT QVFV DTVG MPET YQAR LQQS FPGI EVTV KAKA DALY PVVS AASI CAKV ARDQ AVKK WQFV EKLQ DLDT DYGS GYPN DPKT KAWL KEHV EPVF GFPQ FVRF SWRT AQTI LEKE AEDV IWED SASE NQEG LRKI TSYF LNEG SQAR PRSS HRYF LERG LESA TSL
Gene ID:	10535
Uniprot:	O75792
Cellular Location:	Nucleus
Calculated MW:	33kDa
Observed MW:	33kDa

Synonyms:

RNASEH2A, AGS4, JUNB, RNASEHI, RNHIA, RNHL

Background:

The protein encoded by this gene is a component of the heterotrimeric type II ribonuclease H enzyme (RNAseH2). RNAseH2 is the major source of ribonuclease H activity in mammalian cells and endonucleolytically cleaves ribonucleotides. It is predicted to remove Okazaki fragment RNA primers during lagging strand DNA synthesis and to excise single ribonucleotides from DNA-DNA duplexes. Mutations in this gene cause Aicardi-Goutieres Syndrome (AGS), a an autosomal recessive neurological disorder characterized by progressive microcephaly and psychomotor retardation, intracranial calcifications, elevated levels of interferon-alpha and white blood cells in the cerebrospinal fluid.

UniProt Protein Function:	RNASEH2A: Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging- strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes. Defects in RNASEH2A are the cause of Aicardi-Goutieres syndrome type 4 (AGS4). A form of Aicardi-Goutieres syndrome, a genetically heterogeneous disease characterized by cerebral atrophy, leukoencephalopathy, intracranial calcifications, chronic cerebrospinal fluid (CSF) lymphocytosis, increased CSF alpha-interferon, and negative serologic investigations for common prenatal infection. Clinical features as thrombocytopenia, hepatosplenomegaly and elevated hepatic transaminases along with intermittent fever may erroneously suggest an infective process. Severe neurological dysfunctions manifest in infancy as progressive microcephaly, spasticity, dystonic posturing and profound psychomotor retardation. Death often occurs in early childhood. Belongs to the RNase HII family. Eukaryotic subfamily.
UniProt Protein Details:	Protein type:Ribonuclease; EC 3.1.26.4; DNA replication Chromosomal Location of Human Ortholog: 19p13.2 Cellular Component: nucleus Molecular Function:RNA binding; metal ion binding; ribonuclease H activity; ribonuclease activity Biological Process: mismatch repair; RNA catabolic process; DNA replication Disease: Aicardi-goutieres Syndrome 4
NCBI Summary:	The protein encoded by this gene is a component of the heterotrimeric type II ribonuclease H enzyme (RNAseH2). RNAseH2 is the major source of ribonuclease H activity in mammalian cells and endonucleolytically cleaves ribonucleotides. It is predicted to remove Okazaki fragment RNA primers during lagging strand DNA synthesis and to excise single ribonucleotides from DNA-DNA duplexes. Mutations in this gene cause Aicardi-Goutieres Syndrome (AGS), a an autosomal recessive neurological disorder characterized by progressive microcephaly and psychomotor retardation, intracranial calcifications, elevated levels of interferon-alpha and white blood cells in the cerebrospinal fluid.[provided by RefSeq, Aug 2009]
UniProt Code:	O75792
NCBI GenInfo Identifier:	20981704
NCBI Gene ID:	10535
NCBI Accession:	O75792.2
UniProt Related Accession:	O75792,A0A1W2PP97
Molecular Weight:	33kDa
NCBI Full Name:	Ribonuclease H2 subunit A
NCBI Synonym Full Names:	ribonuclease H2 subunit A
NCBI Official Symbol:	RNASEH2A
NCBI Official Synonym Symbols:	AGS4; JUNB; RNHL; RNHIA; THSD8; RNASEHI
NCBI Protein Information:	ribonuclease H2 subunit A
UniProt Protein Name:	Ribonuclease H2 subunit A
UniProt Synonym Protein Names:	Aicardi-Goutieres syndrome 4 protein; AGS4; RNase H(35); Ribonuclease HI large subunit; RNase HI large subunit; Ribonuclease HI subunit A
Protein Family:	Ribonuclease
UniProt Gene Name:	RNASEH2A
UniProt Entry Name:	RNH2A_HUMAN

	Western blot analysis of extracts of various cell lines, using RNASEH2A antibody (CAB15132) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of C6 cells using RNASEH2A antibody (CAB15132) at dilution of 1:100. Blue: DAPI for nuclear staining.