KO Validated Antibodies 1

[KO Validated] RhoGDI Rabbit Monoclonal Antibody (CAB11556)

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SKU:
CAB11556
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
GDIA1, NPHS8, RHOGDI, RHOGDI-1, HEL-S-47e, DI
Research Use:
Cell Biology
Frequently bought together:

Description

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[KO Validated] RhoGDI Monoclonal Antibody (CAB11556)

The [KO Validated] RhoGDI Monoclonal Antibody (CAB11556) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated specifically for use in knockout validation studies, is highly specific and sensitive for detecting RhoGDI in various experimental settings.RhoGDI, a guanine nucleotide dissociation inhibitor, plays a key role in maintaining the proper localization and activity of Rho family GTPases, which are essential for diverse cellular processes such as cytoskeletal organization and cell motility.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] RhoGDI Monoclonal Antibody
SKU:CAB11556
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Clone Number:ARC0629
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:VVVT GLTL VCSS APGP LELD LTGD LESF KKQS FVLK EGVE YRIK ISFR VNRE IVSG MKYI QHTY RKGV KIDK TDYM VGSY G
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:1000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:GDIA1, NPHS8, RHOGDI, RHOGDI-1, HEL-S-47e, DI
Positive Sample:HeLa, 293T, NIH/3T3, U-251MG, Mouse brain, Rat liver
Cellular Localization:Cytoplasm.
Calculated MW:23kDa
Observed MW:26kDa

This gene encodes a protein that plays a key role in the regulation of signaling through Rho GTPases. The encoded protein inhibits the disassociation of Rho family members from GDP (guanine diphosphate), thereby maintaining these factors in an inactive state. Activity of this protein is important in a variety of cellular processes, and expression of this gene may be altered in tumors. Mutations in this gene have been found in individuals with nephrotic syndrome, type 8. Alternate splicing results in multiple transcript variants.

Purification MethodAffinity purification
Gene ID396
RRIDAB_2861595
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using RhoGDI Rabbit mAb (CAB11556) at 1：1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageWestern blot analysis of lysates from wild type (WT) and RhoGDI knockout (KO) 293T cells, using [KO Validated] RhoGDI Rabbit mAb (CAB11556) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
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