Cell Biology Antibodies 2
Anti-RHEB Antibody (CAB1165)
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- SKU:
- CAB1165
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-RHEB Antibody |
| Antibody SKU: | CAB1165 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-184 of human RHEB (NP_005605.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, Raji, HT-29 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-184 of human RHEB (NP_005605.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MPQS KSRK IAIL GYRS VGKS SLTI QFVE GQFV DSYD PTIE NTFT KLIT VNGQ EYHL QLVD TAGQ DEYS IFPQ TYSI DING YILV YSVT SIKS FEVI KVIH GKLL DMVG KVQI PIML VGNK KDLH MERV ISYE EGKA LAES WNAA FLES SAKE NQTA VDVF RRII LEAE KMDG AASQ GKSS CSVM |
| Gene ID: | 6009 |
| Uniprot: | Q15382 |
| Cellular Location: | Cytoplasm, Cytoplasmic side, Endomembrane system, Endoplasmic reticulum membrane, Golgi apparatus membrane, Lipid-anchor, cytosol |
| Calculated MW: | 20kDa |
| Observed MW: | 16kDa |
| Synonyms: | RHEB, RHEB2 |
| Background: | This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22. |
| UniProt Protein Function: | RHEB: Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity. Binds to mTORC1 in a guanyl nucleotide-independent manner. Interacts directly with MTOR, MLST8 and RPTOR. Interacts with TSC2. Ubiquitous. Highest levels observed in skeletal and cardiac muscle. Alternates between an inactive form bound to GDP and an active form bound to GTP. Inactivated by TSC1-TSC2 via the GTPase activating protein (GAP) domain of TSC2. Belongs to the small GTPase superfamily. Rheb family. |
| UniProt Protein Details: | Protein type:G protein; G protein, monomeric; G protein, monomeric, Rheb Chromosomal Location of Human Ortholog: 7q36 Cellular Component: Golgi membrane; spliceosome; endoplasmic reticulum membrane; membrane; cell soma; dendrite; plasma membrane; cytosol Molecular Function:GTPase activity; protein binding; GDP binding; GTP binding; metal ion binding; protein kinase binding Biological Process: metabolic process; small GTPase mediated signal transduction; insulin receptor signaling pathway; signal transduction; cell cycle arrest; positive regulation of TOR signaling pathway |
| NCBI Summary: | This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q15382 |
| NCBI GenInfo Identifier: | 6919957 |
| NCBI Gene ID: | 6009 |
| NCBI Accession: | Q15382.1 |
| UniProt Secondary Accession: | Q15382,Q53Y56, Q99444, B3KWN6, D3DX13, |
| UniProt Related Accession: | Q15382 |
| Molecular Weight: | 184 |
| NCBI Full Name: | GTP-binding protein Rheb |
| NCBI Synonym Full Names: | Ras homolog enriched in brain |
| NCBI Official Symbol: | RHEB |
| NCBI Official Synonym Symbols: | RHEB2 |
| NCBI Protein Information: | GTP-binding protein Rheb; Ras homolog enriched in brain 2 |
| UniProt Protein Name: | GTP-binding protein Rheb |
| UniProt Synonym Protein Names: | Ras homolog enriched in brain |
| Protein Family: | Rheb-like protein |
| UniProt Gene Name: | RHEB |
| UniProt Entry Name: | RHEB_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RHEB antibody (CAB1165) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
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