Name: Anti-RGR Antibody (CAB7925)
Brand: Signal Transduction Antibodies 3
SKU: CAB7925
Availability: OutOfStock

Antibody Name:	Anti-RGR Antibody
Antibody SKU:	CAB7925
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 156-295 of human RGR (NP_002912.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Rat
Positive Samples:	rat spinal cord

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 156-295 of human RGR (NP_002912.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	HYDY EPLG TCCT LDYS KGDR NFTS FLFT MSFF NFAM PLFI TITS YSLM EQKL GKSG HLQV NTTL PART LLLG WGPY AILY LYAV IADV TSIS PKLQ MVPA LIAK MVPT INAI NYAL GNEM VCRG IWQC LSPQ KREK DRTK
Gene ID:	5995
Uniprot:	P47804
Cellular Location:	Membrane, Multi-pass membrane protein
Calculated MW:	27kDa/31kDa/32kDa
Observed MW:	32kDa

Synonyms:

RGR, RP44

Background:

This gene encodes a putative retinal G-protein coupled receptor. The gene is a member of the opsin subfamily of the 7 transmembrane, G-protein coupled receptor 1 family. Like other opsins which bind retinaldehyde, it contains a conserved lysine residue in the seventh transmembrane domain. The protein acts as a photoisomerase to catalyze the conversion of all-trans-retinal to 11-cis-retinal. The reverse isomerization occurs with rhodopsin in retinal photoreceptor cells. The protein is exclusively expressed in tissue adjacent to retinal photoreceptor cells, the retinal pigment epithelium and Mueller cells. This gene may be associated with autosomal recessive and autosomal dominant retinitis pigmentosa (arRP and adRP, respectively). Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	RGR: Receptor for all-trans- and 11-cis-retinal. Binds preferentially to the former and may catalyze the isomerization of the chromophore by a retinochrome-like mechanism. Defects in RGR are the cause of retinitis pigmentosa type 44 (RP44). RP44 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. Belongs to the G-protein coupled receptor 1 family. Opsin subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:GPCR, family 1; Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 10q23 Cellular Component: integral to plasma membrane Molecular Function:G-protein coupled receptor activity; protein binding; chemokine receptor activity; photoreceptor activity Biological Process: G-protein coupled receptor protein signaling pathway; visual perception; phototransduction; chemotaxis; protein-chromophore linkage Disease: Retinitis Pigmentosa 44
NCBI Summary:	This gene encodes a putative retinal G-protein coupled receptor. The gene is a member of the opsin subfamily of the 7 transmembrane, G-protein coupled receptor 1 family. Like other opsins which bind retinaldehyde, it contains a conserved lysine residue in the seventh transmembrane domain. The protein acts as a photoisomerase to catalyze the conversion of all-trans-retinal to 11-cis-retinal. The reverse isomerization occurs with rhodopsin in retinal photoreceptor cells. The protein is exclusively expressed in tissue adjacent to retinal photoreceptor cells, the retinal pigment epithelium and Mueller cells. This gene may be associated with autosomal recessive and autosomal dominant retinitis pigmentosa (arRP and adRP, respectively). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:	P47804
NCBI GenInfo Identifier:	1350592
NCBI Gene ID:	5995
NCBI Accession:	P47804.1
UniProt Secondary Accession:	P47804,Q96FC5, A6NKK7,
UniProt Related Accession:	P47804
Molecular Weight:	291
NCBI Full Name:	RPE-retinal G protein-coupled receptor
NCBI Synonym Full Names:	retinal G protein coupled receptor
NCBI Official Symbol:	RGR
NCBI Official Synonym Symbols:	RP44
NCBI Protein Information:	RPE-retinal G protein-coupled receptor; RGR-opsin; RPE retinal G-protein coupled receptor
UniProt Protein Name:	RPE-retinal G protein-coupled receptor
Protein Family:	RPE-retinal G protein-coupled receptor
UniProt Gene Name:	RGR
UniProt Entry Name:	RGR_HUMAN

Western blot analysis of extracts of rat spinal cord, using RGR antibody (CAB7925) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

RGR Antibody (PACO04919)

RGR Antibody (PACO11815)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)