Developmental Biology

Anti-RFX3 Antibody (CAB15311)

(No reviews yet) Write a Review
SKU:
CAB15311
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Developmental Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-RFX3 Antibody
Antibody SKU:CAB15311
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 280-430 of human RFX3 (NP_602304.1).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human
Positive Samples:293T, HeLa, K-562
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 280-430 of human RFX3 (NP_602304.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:QQKQ RYKP MQKV DGVA DGFT GSGQ QTGT SVEQ TVIA QSQH HQQF LDAS RALP EFGE VEIS SLPD GTTF EDIK SLQS LYRE HCEA ILDV VVNL QFSL IEKL WQTF WRYS PSTP TDGT TITE SSNL SEIE SRLP KAKL ITLC KHES ILKW MCN
Gene ID:5991
Uniprot:P48380
Cellular Location:Nucleus
Calculated MW:45kDa/79kDa/83kDa
Observed MW:84kDa
Synonyms:RFX3
Background:This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1, X2, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with other RFX family members. Multiple transcript variants encoding different isoforms have been described for this gene.
UniProt Protein Function:RFX3: Transcription factor required for ciliogenesis and islet cell differentiation during endocrine pancreas development. Essential for the differentiation of nodal monocilia and left- right asymmetry specification during embryogenesis. Required for the biogenesis of motile cilia by governing growth and beating efficiency of motile cells. Also required for ciliated ependymal cell differentiation. Regulates the expression of genes involved in ciliary assembly (DYNC2LI1, FOXJ1 and BBS4) and genes involved in ciliary motility (DNAH11, DNAH9 and DNAH5). Together with RFX6, participates in the differentiation of 4 of the 5 islet cell types during endocrine pancreas development, with the exception of pancreatic PP (polypeptide-producing) cells. Regulates transcription by forming a heterodimer with another RFX protein and binding to the X-box in the promoter of target genes. Acts as a transcription factor. Represses transcription of MAP1A in non-neuronal cells but not in neuronal cells. Belongs to the RFX family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transcription, coactivator/corepressor; DNA-binding

Chromosomal Location of Human Ortholog: 9p24.2

Cellular Component: nuclear chromatin; nucleus; transcription factor complex

Molecular Function:DNA binding; protein binding; RNA polymerase II transcription factor activity, enhancer binding

Biological Process: cell maturation; cilium biogenesis; endocrine pancreas development; negative regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of insulin secretion; regulation of transcription, DNA-dependent

NCBI Summary:This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1, X2, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with other RFX family members. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2013]
UniProt Code:P48380
NCBI GenInfo Identifier:32172437
NCBI Gene ID:5991
NCBI Accession:P48380.2
UniProt Secondary Accession:P48380,Q5JTL7, Q5JTL8, Q6NW13, Q8WTU4, Q95HL5, Q95HL6 A8K0H5, D3DRH8, D3DRH9,
UniProt Related Accession:P48380
Molecular Weight:45,715 Da
NCBI Full Name:Transcription factor RFX3
NCBI Synonym Full Names:regulatory factor X3
NCBI Official Symbol:RFX3  
NCBI Protein Information:transcription factor RFX3
UniProt Protein Name:Transcription factor RFX3
UniProt Synonym Protein Names:Regulatory factor X 3
Protein Family:Transcription factor
UniProt Gene Name:RFX3  
UniProt Entry Name:RFX3_HUMAN
Anti-RFX3 Antibody (CAB15311)Western blot analysis of extracts of various cell lines, using RFX3 antibody (CAB15311) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
View AllClose

0 Reviews

View AllClose

Related Products