Signal Transduction Antibodies 3

Anti-RFK Antibody (CAB9141)

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SKU:
CAB9141
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-RFK Antibody
Antibody SKU:CAB9141
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-155 of human RFK (NP_060809.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:U-87MG, HeLa, 293T, Mouse small intestine, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-155 of human RFK (NP_060809.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MRHL PYFC RGQV VRGF GRGS KQLG IPTA NFPE QVVD NLPA DIST GIYY GWAS VGSG DVHK MVVS IGWN PYYK NTKK SMET HIMH TFKE DFYG EILN VAIV GYLR PEKN FDSL ESLI SAIQ GDIE EAKK RLEL PEHL KIKE DNFF QVSK SKIM NGH
Gene ID:55312
Uniprot:Q969G6
Cellular Location:Cytoplasm
Calculated MW:17kDa
Observed MW:14kDa
Synonyms:RFK, RIFK
Background:
UniProt Protein Function:RFK: Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN).
UniProt Protein Details:

Protein type:Kinase, other; EC 2.7.1.26; Cofactor and Vitamin Metabolism - riboflavin

Chromosomal Location of Human Ortholog: 9q21.13

Cellular Component: cytosol

Molecular Function:riboflavin kinase activity

Biological Process: apoptosis; positive regulation of NAD(P)H oxidase activity; riboflavin metabolic process

NCBI Summary:Riboflavin kinase (RFK; EC 2.7.1.26) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis (Karthikeyan et al., 2003 [PubMed 12623014]).[supplied by OMIM, Nov 2009]
UniProt Code:Q969G6
NCBI GenInfo Identifier:209572667
NCBI Gene ID:55312
NCBI Accession:Q969G6.2
UniProt Secondary Accession:Q969G6,Q5JSG9, Q9NUT7,
UniProt Related Accession:Q969G6
Molecular Weight:17,623 Da
NCBI Full Name:Riboflavin kinase
NCBI Synonym Full Names:riboflavin kinase
NCBI Official Symbol:RFK  
NCBI Official Synonym Symbols:RIFK  
NCBI Protein Information:riboflavin kinase
UniProt Protein Name:Riboflavin kinase
UniProt Synonym Protein Names:ATP:riboflavin 5'-phosphotransferase; Flavokinase
UniProt Gene Name:RFK  
UniProt Entry Name:RIFK_HUMAN
Anti-RFK Polyclonal Antibody (CAB9141)Western blot analysis of extracts of various cell lines, using RFK antibody (CAB9141) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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