Name: Anti-RFC2 Antibody (CAB6847)
Brand: Epigenetics & Nuclear Signaling Antibodies 4
SKU: CAB6847
Availability: OutOfStock

Antibody Name:	Anti-RFC2 Antibody
Antibody SKU:	CAB6847
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 38-297 of human RFC2 (NP_852136.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	LO2, 22Rv1, Mouse spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 38-297 of human RFC2 (NP_852136.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	WVEK YRPV KLNE IVGN EDTV SRLE VFAR EGNV PNII IAGP PGTG KTTS ILCL ARAL LGPA LKDA MLEL NASN DRGI DVVR NKIK MFAQ QKVT LPKG RHKI IILD EADS MTDG AQQA LRRT MEIY SKTT RFAL ACNA SDKI IEPI QSRC AVLR YTKL TDAQ ILTR LMNV IEKE RVPY TDDG LEAI IFTA QGDM RQAL NNLQ STFS GFGF INSE NVFK VCDE PHPL LVKE MIQH CVNA NIDE AYKI LAHL WHLG YSPE DIIG
Gene ID:	5982
Uniprot:	P35250
Cellular Location:	Nucleus
Calculated MW:	35kDa/39kDa
Observed MW:	39kDa

Synonyms:

RFC2, RFC40

Background:

This gene encodes a member of the activator 1 small subunits family. The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). Replication factor C, also called activator 1, is a protein complex consisting of five distinct subunits. This gene encodes the 40 kD subunit, which has been shown to be responsible for binding ATP and may help promote cell survival. Disruption of this gene is associated with Williams syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been described. A pseudogene of this gene has been defined on chromosome 2.

UniProt Protein Function:	RFC2: The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit binds ATP. RFC2 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Belongs to the activator 1 small subunits family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:DNA replication Chromosomal Location of Human Ortholog: 7q11.23 Cellular Component: DNA replication factor C complex; nucleoplasm Molecular Function:DNA clamp loader activity; protein binding; single-stranded DNA-dependent ATPase activity Biological Process: bypass DNA synthesis; DNA damage response, detection of DNA damage; DNA replication; error-prone postreplication DNA repair; nucleotide-excision repair, DNA gap filling; nucleotide-excision repair, DNA incision; nucleotide-excision repair, DNA incision, 5'-to lesion; telomere maintenance via recombination; transcription-coupled nucleotide-excision repair
NCBI Summary:	This gene encodes a member of the activator 1 small subunits family. The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). Replication factor C, also called activator 1, is a protein complex consisting of five distinct subunits. This gene encodes the 40 kD subunit, which has been shown to be responsible for binding ATP and may help promote cell survival. Disruption of this gene is associated with Williams syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been described. A pseudogene of this gene has been defined on chromosome 2. [provided by RefSeq, Jul 2013]
UniProt Code:	P35250
NCBI GenInfo Identifier:	2507300
NCBI Gene ID:	5982
NCBI Accession:	P35250.3
UniProt Secondary Accession:	P35250,P32846, Q9BU93, B5BU07, D3DXG3,
UniProt Related Accession:	P35250
Molecular Weight:	35,244 Da
NCBI Full Name:	Replication factor C subunit 2
NCBI Synonym Full Names:	replication factor C subunit 2
NCBI Official Symbol:	RFC2
NCBI Official Synonym Symbols:	RFC40
NCBI Protein Information:	replication factor C subunit 2
UniProt Protein Name:	Replication factor C subunit 2
UniProt Synonym Protein Names:	Activator 1 40 kDa subunit; A1 40 kDa subunit; Activator 1 subunit 2; Replication factor C 40 kDa subunit; RF-C 40 kDa subunit; RFC40
Protein Family:	Replication factor
UniProt Gene Name:	RFC2
UniProt Entry Name:	RFC2_HUMAN

Western blot analysis of extracts of various cell lines, using RFC2 antibody (CAB6847) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

Antibodies

RFC2 Antibody (PACO04917)

RFC2 Antibody (PACO11804)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)