Epigenetics & Nuclear Signaling Antibodies 5

Anti-REST / NRSF Antibody (CAB2415)

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SKU:
CAB2415
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-REST / NRSF Antibody
Antibody SKU:CAB2415
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human REST / NRSF
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:Rat brain, HeLa, U-87MG, Mouse brain
Immunogen:A synthesized peptide derived from human REST / NRSF
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:5978
Uniprot:Q13127
Cellular Location:
Calculated MW:160kDa
Observed MW:170KDa
Synonyms:NRSF, WT6, XBR
Background:This gene encodes a transcriptional repressor that represses neuronal genes in non-neuronal tissues. It is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regular of neurogenesis. Alternatively spliced transcript variants have been described [provided by RefSeq, Jul 2010]
UniProt Protein Function:REST: a transcriptional repressor that binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells during embryonic development. Induced during ageing in human cortical and hippocampal neurons, where it represses genes that promote cell death and induces the expression of stress response genes. Protects neurons from amyloid beta- and oxidative stress-induced toxicity. The deletion of REST in the mouse brain induces age-related neurodegeneration. Restricts the expression of neuronal genes by associating with two distinct corepressors, SIN3A and RCOR1, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. REST activates DYRK1A transcription via a neuron-restricted silencer element in the DYRK1A promoter. Four isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transcription factor; DNA-binding; C2H2-type zinc finger protein; Cell development/differentiation

Chromosomal Location of Human Ortholog: 4q12

Cellular Component: nucleoplasm; transcriptional repressor complex; cytosol; nucleus

Molecular Function:protein binding; metal ion binding; outward rectifier potassium channel activity; chromatin binding; transcription factor activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; negative regulation of calcium ion-dependent exocytosis; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; positive regulation of caspase activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of insulin secretion; negative regulation of neurogenesis; negative regulation of cell proliferation; negative regulation of neuron differentiation; regulation of transcription, DNA-dependent; negative regulation of aldosterone biosynthetic process; hemopoietic progenitor cell differentiation; negative regulation of transcription, DNA-dependent

NCBI Summary:This gene encodes a transcriptional repressor that represses neuronal genes in non-neuronal tissues. It is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regular of neurogenesis. Alternatively spliced transcript variants have been described [provided by RefSeq, Jul 2010]
UniProt Code:Q13127
NCBI GenInfo Identifier:296452989
NCBI Gene ID:5978
NCBI Accession:Q13127.3
UniProt Secondary Accession:Q13127,Q12956, Q12957, Q13134, Q59ER1, Q8IWI3, A2RUE0 B9EGJ0,
UniProt Related Accession:Q13127
Molecular Weight:1097
NCBI Full Name:RE1-silencing transcription factor
NCBI Synonym Full Names:RE1-silencing transcription factor
NCBI Official Symbol:REST  
NCBI Official Synonym Symbols:XBR; NRSF  
NCBI Protein Information:RE1-silencing transcription factor; repressor binding to the X2 box; neural-restrictive silencer factor; neuron restrictive silencer factor; RE1-silencing transcription factor variant E1a/E2/E4; RE1-silencing transcription factor variant E1a/E2/E3/E5; RE1-silencing transcription factor variant E1b/E2/E3/E5; RE1-silencing transcription factor variant E1c/E2/E3/E5; RE1-silencing transcription factor variant E1a/E2/E3/E4c; RE1-silencing transcription factor variant E1c/E2g/E3/E4; RE1-silencing transcription factor variant E1a/E2/E3/N3c/E4; RE1-silencing transcription factor variant E1b/E2/E3/N3c/E4; RE1-silencing transcription factor variant E1a/E2/E3/N3a/E4i; RE1-silencing transcription factor variant E1b/E2/E3/N3b/E4i; RE1-silencing transcription factor variant E1b/E2c/E2j/E3/E4; RE1-silencing transcription factor variant E1a/E2k/E2i/E3/E4j
UniProt Protein Name:RE1-silencing transcription factor
UniProt Synonym Protein Names:Neural-restrictive silencer factor; X2 box repressor
Protein Family:Restin
UniProt Gene Name:REST  
UniProt Entry Name:REST_HUMAN
Anti-REST / NRSF Antibody (CAB2415)Western blot - REST / NRSF Rabbit mAb (CAB2415)
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