Metabolism Antibodies 2
Anti-RECK Antibody (CAB6718)
- SKU:
- CAB6718
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-RECK Antibody |
| Antibody SKU: | CAB6718 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 110-210 of human RECK (NP_066934.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, MCF-7, H460, Mouse lung, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 110-210 of human RECK (NP_066934.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ALEC RQAC KQAS SKND ISKV CRKE YENA LFSC ISRN EMGS VCCS YAGH HTNC REYC QAIF RTDS SPGP SQIK AVEN YCAS ISPQ LIHC VNNY TQSY PMRN P |
| Gene ID: | 8434 |
| Uniprot: | O95980 |
| Cellular Location: | Cell membrane, GPI-anchor, Lipid-anchor |
| Calculated MW: | 106kDa |
| Observed MW: | 106kDa |
| Synonyms: | RECK, ST15 |
| Background: | The protein encoded by this gene is a cysteine-rich, extracellular protein with protease inhibitor-like domains whose expression is suppressed strongly in many tumors and cells transformed by various kinds of oncogenes. In normal cells, this membrane-anchored glycoprotein may serve as a negative regulator for matrix metalloproteinase-9, a key enzyme involved in tumor invasion and metastasis. Several transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | RECK: Negatively regulates matrix metalloproteinase-9 (MMP-9) by suppressing MMP-9 secretion and by direct inhibition of its enzymatic activity. RECK down-regulation by oncogenic signals may facilitate tumor invasion and metastasis. Appears to also regulate MMP-2 and MT1-MMP, which are involved in cancer progression. Interacts with MMP-9. Expressed in various tissues and untransformed cells. It is undetectable in tumor-derived cell lines and oncogenically transformed cells. |
| UniProt Protein Details: | Protein type:Membrane protein, GPI anchor; Tumor suppressor Chromosomal Location of Human Ortholog: 9p13.3 Cellular Component: membrane; plasma membrane Molecular Function:serine-type endopeptidase inhibitor activity; metalloendopeptidase inhibitor activity; protein binding; endopeptidase inhibitor activity Biological Process: extracellular matrix organization and biogenesis; blood vessel maturation; embryo implantation |
| NCBI Summary: | The protein encoded by this gene is a cysteine-rich, extracellular protein with protease inhibitor-like domains whose expression is suppressed strongly in many tumors and cells transformed by various kinds of oncogenes. In normal cells, this membrane-anchored glycoprotein may serve as a negative regulator for matrix metalloproteinase-9, a key enzyme involved in tumor invasion and metastasis. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O95980 |
| NCBI GenInfo Identifier: | 20178043 |
| NCBI Gene ID: | 8434 |
| NCBI Accession: | O95980.1 |
| UniProt Secondary Accession: | O95980,Q5W0K6, Q8WX37, B2RNS1, |
| UniProt Related Accession: | O95980 |
| Molecular Weight: | 971 |
| NCBI Full Name: | Reversion-inducing cysteine-rich protein with Kazal motifs |
| NCBI Synonym Full Names: | reversion-inducing-cysteine-rich protein with kazal motifs |
| NCBI Official Symbol: | RECK |
| NCBI Official Synonym Symbols: | ST15 |
| NCBI Protein Information: | reversion-inducing cysteine-rich protein with Kazal motifs; suppressor of tumorigenicity 15 protein; membrane-anchored glycoprotein (metastasis and invasion); suppression of tumorigenicity 5 (reversion-inducing-cysteine-rich protein with kazal motifs); suppression of tumorigenicity 15 (reversion-inducing-cysteine-rich protein with kazal motifs) |
| UniProt Protein Name: | Reversion-inducing cysteine-rich protein with Kazal motifs |
| UniProt Synonym Protein Names: | Suppressor of tumorigenicity 15 protein |
| Protein Family: | Reversion-inducing cysteine-rich protein |
| UniProt Gene Name: | RECK |
| UniProt Entry Name: | RECK_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RECK antibody (CAB6718) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s. |
 | Immunofluorescence analysis of C6 cells using RECK Polyclonal Antibody (CAB6718) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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