Name: Anti-RBX1 Antibody (CAB14500)
Brand: Epigenetics & Nuclear Signaling Antibodies 3
SKU: CAB14500
Availability: OutOfStock

Antibody Name:	Anti-RBX1 Antibody
Antibody SKU:	CAB14500
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RBX1 (NP_055063.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Mouse, Rat
Positive Samples:	Mouse testis, Rat brain, Rat testis

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RBX1 (NP_055063.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAA MDVD TPSG TNSG AGKK RFEV KKWN AVAL WAWD IVVD NCAI CRNH IMDL CIEC QANQ ASAT SEEC TVAW GVCN HAFH FHCI SRWL KTRQ VCPL DNRE
Gene ID:	9978
Uniprot:	P62877
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	12kDa
Observed MW:	12kDa

Synonyms:	RBX1, BA554C12.1, RNF75, ROC1
Background:	This locus encodes a RING finger-like domain-containing protein. The encoded protein interacts with cullin proteins and likely plays a role in ubiquitination processes necessary for cell cycle progression. This protein may also affect protein turnover. Related pseudogenes exist on chromosomes 2 and 5.

UniProt Protein Function:	RBX1: E3 ubiquitin ligase component of multiple cullin-RING- based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M. Part of a SCF complex consisting of CUL1, RBX1, SKP1 and SKP2. Part of a SCF-like complex consisting of CUL7, RBX1, SKP1 and FBXW8. Part of CBC(VHL) complexes with elongin BC complex (TCEB1 and TCEB2), CUL2 or CUL5 and VHL. Part of the CSA complex (DCX(ERCC8) complex), a DCX E3 ubiquitin-protein ligase complex containing ERCC8, RBX1, DDB1 and CUL4A; the CSA complex interacts with RNA polymerase II; upon UV irradiation it interacts with the COP9 signalosome and preferentially with the hyperphosphorylated form of RNA polymerase II. Part of multisubunit E3 ubiquitin ligase complexes with elongin BC complex (TCEB1 and TCEB2), CUL2 and MED8; elongin BC complex (TCEB1 and TCEB2), CUL5 and MUF1. Part of multisubunit complexes with elongin BC complex (TCEB1 and TCEB2), elongin A/TCEB3 or SOCS1 or WSB1 and CUL5. Interacts directly with CUL1 and probably also with CUL2, CUL3, CUL4A, CUL4B, CUL5 and CUL7. Probably interacts with CDC34. Interacts with COPS6. Component of the DCX DET1-COP1 ubiquitin ligase complex at least composed of RBX1, DET1, DDB1, CUL4A and COP1. Part of an E3 ligase complex composed of RBX1, DDB1, DDB2 and CUL4A or CUL4B. Interacts with UBE2M. Part of a SCF complex consisting of CUL1, FBXO3, RBX1 and SKP1; this complex interacts with PML via FBXO3. Interacts with human adenovirus early E1A protein; this interaction inhibits RBX1-CUL1-dependent elongation reaction of ubiquitin chains by the SCF(FBW7) complex. Component of the SCF(Cyclin F) complex consisting of CUL1, RBX1, SKP1 and CCNF. Widely expressed. Belongs to the RING-box family.
UniProt Protein Details:	Protein type:EC 6.3.2.-; Ubiquitin ligase; Ubiquitin conjugating system; Ligase Chromosomal Location of Human Ortholog: 22q13.2 Cellular Component: Cul2-RING ubiquitin ligase complex; nucleoplasm; cullin-RING ubiquitin ligase complex; Cul5-RING ubiquitin ligase complex; VCB complex; SCF ubiquitin ligase complex; cytosol Molecular Function:protein binding; zinc ion binding; ubiquitin protein ligase binding; protein complex binding; ubiquitin-protein ligase activity; NEDD8 ligase activity; ligase activity Biological Process: protein monoubiquitination; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; Notch signaling pathway; protein neddylation; viral reproduction; protein ubiquitination; DNA repair
NCBI Summary:	This locus encodes a RING finger-like domain-containing protein. The encoded protein interacts with cullin proteins and likely plays a role in ubiquitination processes necessary for cell cycle progression. This protein may also affect protein turnover. Related pseudogenes exist on chromosomes 2 and 5.[provided by RefSeq, Sep 2010]
UniProt Code:	P62877
NCBI GenInfo Identifier:	51338609
NCBI Gene ID:	9978
NCBI Accession:	P62877.1
UniProt Related Accession:	P62877
Molecular Weight:
NCBI Full Name:	E3 ubiquitin-protein ligase RBX1
NCBI Synonym Full Names:	ring-box 1
NCBI Official Symbol:	RBX1
NCBI Official Synonym Symbols:	ROC1; RNF75; BA554C12.1
NCBI Protein Information:	E3 ubiquitin-protein ligase RBX1
UniProt Protein Name:	E3 ubiquitin-protein ligase RBX1
UniProt Synonym Protein Names:	Protein ZYP; RING finger protein 75; RING-box protein 1; Rbx1; Regulator of cullins 1E3 ubiquitin-protein ligase RBX1, N-terminally processed
Protein Family:	RING-box protein
UniProt Gene Name:	RBX1
UniProt Entry Name:	RBX1_HUMAN

Western blot analysis of extracts of various cell lines, using RBX1 Rabbit pAb (CAB14500) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.

Antibodies

Anti-RBX1 Antibody (CAB1832)

RBX1 Antibody (PACO11775)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)