KO Validated Antibodies 2

Anti-RBM3 Antibody (CAB19967)[KO Validated]

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SKU:
CAB19967
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism

Description

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Antibody Name:Anti-RBM3 Antibody [KO Validated]
Antibody SKU:CAB19967
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human RBM3.
Application:WB IHC IF
Recommended Dilution:WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T
Immunogen:Recombinant protein of human RBM3.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:5935
Uniprot:P98179
Cellular Location:Cell projection, Cytoplasm, Nucleus, dendrite
Calculated MW:17kDa
Observed MW:17KDa
Anti-RBM3 Antibody [KO Validated] (CAB19967)Western blot analysis of extracts from normal (control) and RBM3 knockout (KO) 293T cells, using RBM3 antibody (CAB19967) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
Synonyms:RBM3, IS1-RNPL, RNPL
Background:This gene is a member of the glycine-rich RNA-binding protein family and encodes a protein with one RNA recognition motif (RRM) domain. Expression of this gene is induced by cold shock and low oxygen tension. A pseudogene exists on chromosome 1. Multiple alternatively spliced transcript variants that are predicted to encode different isoforms have been characterized although some of these variants fit nonsense-mediated decay (NMD) criteria.
UniProt Protein Function:RBM3: Cold-inducible mRNA binding protein that enhances global protein synthesis at both physiological and mild hypothermic temperatures. Reduces the relative abundance of microRNAs, when overexpressed. Enhances phosphorylation of translation initiation factors and active polysome formation.
UniProt Protein Details:

Protein type:Translation; RNA-binding; Ribosomal; RNA processing

Chromosomal Location of Human Ortholog: Xp11.2

Cellular Component: cytoplasm; dendrite; nucleus; large ribosomal subunit

Molecular Function:protein binding; RNA binding; nucleotide binding; ribosomal large subunit binding

Biological Process: RNA processing; regulation of translation; miRNA-mediated gene silencing, production of miRNAs; positive regulation of translation; translation; response to cold

NCBI Summary:This gene is a member of the glycine-rich RNA-binding protein family and encodes a protein with one RNA recognition motif (RRM) domain. Expression of this gene is induced by cold shock and low oxygen tension. A pseudogene exists on chromosome 1. Multiple alternatively spliced transcript variants that are predicted to encode different isoforms have been characterized although some of these variants fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq, Jul 2008]
UniProt Code:P98179
NCBI GenInfo Identifier:1710620
NCBI Gene ID:5935
NCBI Accession:P98179.1
UniProt Related Accession:P98179
Molecular Weight:157
NCBI Full Name:Putative RNA-binding protein 3
NCBI Synonym Full Names:RNA binding motif (RNP1, RRM) protein 3
NCBI Official Symbol:RBM3  
NCBI Official Synonym Symbols:RNPL; IS1-RNPL  
NCBI Protein Information:putative RNA-binding protein 3; RNA-binding motif protein 3
UniProt Protein Name:Putative RNA-binding protein 3
UniProt Synonym Protein Names:RNA-binding motif protein 3; RNPL
Protein Family:RNA-binding protein
UniProt Gene Name:RBM3  
UniProt Entry Name:RBM3_HUMAN
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