Developmental Biology
Anti-RBM11 Antibody (CAB13216)
- SKU:
- CAB13216
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Description
| Antibody Name: | Anti-RBM11 Antibody |
| Antibody SKU: | CAB13216 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-281 of human RBM11 (NP_658983.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, 293T, MCF7, Mouse testis, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-281 of human RBM11 (NP_658983.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MFPA QEEA DRTV FVGN LEAR VREE ILYE LFLQ AGPL TKVT ICKD REGK PKSF GFVC FKHP ESVS YAIA LLNG IRLY GRPI NVQY RFGS SRSS EPAN QSFE SCVK INSH NYRN EEML VGRS SFPM QYFP INNT SLPQ EYFL FQKM QWHV YNPV LQLP YYEM TAPL PNSA SVSS SLNH VPDL EAGP SSYK WTHQ QPSD SDLY QMTA PLPN SASV SSSL NHVP DLEA GPSS YKWT HQQP SDSD LYQM NKRK RQKQ TSDS DSST DNNR GNEC SQKF RKSK KKKR Y |
| Gene ID: | 54033 |
| Uniprot: | P57052 |
| Cellular Location: | Nucleus, Nucleus speckle, nucleoplasm |
| Calculated MW: | 26kDa/32kDa |
| Observed MW: | 32kDa |
| Synonyms: | RBM11 |
| Background: |
| UniProt Protein Function: | RBM11: Tissue-specific splicing factor with potential implication in the regulation of alternative splicing during neuron and germ cell differentiation. Antagonizes SRSF1-mediated BCL-X splicing. May affect the choice of alternative 5' splice sites by binding to specific sequences in exons and antagonizing the SR protein SRSF1. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 21q11 Cellular Component: nuclear speck; nucleoplasm; nucleus Molecular Function:poly(U) binding; protein binding; protein homodimerization activity Biological Process: regulation of alternative nuclear mRNA splicing, via spliceosome |
| UniProt Code: | P57052 |
| NCBI GenInfo Identifier: | 9978670 |
| NCBI Gene ID: | 54033 |
| NCBI Accession: | P57052.1 |
| UniProt Secondary Accession: | P57052,Q6YNC2, Q8NBA1, Q8NFF6, |
| UniProt Related Accession: | P57052 |
| Molecular Weight: | 26,465 Da |
| NCBI Full Name: | Splicing regulator RBM11 |
| NCBI Synonym Full Names: | RNA binding motif protein 11 |
| NCBI Official Symbol: | RBM11 |
| NCBI Protein Information: | splicing regulator RBM11 |
| UniProt Protein Name: | Splicing regulator RBM11 |
| UniProt Synonym Protein Names: | RNA-binding motif protein 11 |
| Protein Family: | Splicing regulator |
| UniProt Gene Name: | RBM11 |
| UniProt Entry Name: | RBM11_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RBM11 antibody (CAB13216) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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