Anti-RBFOX2 Antibody (CAB20810)

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SKU:
CAB20810
Product Type:
Antibody
Antibody Type:
Secondary Antibody
Host Species:
Mouse
Isotype:
IgG

Description

Antibody Name:Anti-RBFOX2 Antibody (CAB20810)
Antibody SKU:CAB20810
Antibody Size:50µL, 100µL
Application:Western blotting
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human RBFOX2 (NP_001026865.1).
Application:Western blotting
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:U-87MG, LO2, HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human RBFOX2 (NP_001026865.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MEKK KMVT QGNQ EPTT TPDA MVQP FTTI PFPP PPQN GIPT EYGV PHTQ DYAG QTGE HNLT LYGS TQAH GEQS SNSP STQN GSLT TEGG AQTD GQQS QTQS SENS ESKS TP
Cellular Location:
Calculated MW:41kDa
Observed MW:50KDa/60KDa
Synonyms:RTA, fxh, FOX2, RBM9, Fox-2, HNRBP2, HRNBP2, dJ106I20.3
Background:This gene is one of several human genes similar to the C. elegans gene Fox-1. This gene encodes an RNA binding protein that is thought to be a key regulator of alternative exon splicing in the nervous system and other cell types. The protein binds to a conserved UGCAUG element found downstream of many alternatively spliced exons and promotes inclusion of the alternative exon in mature transcripts. The protein also interacts with the estrogen receptor 1 transcription factor and regulates estrogen receptor 1 transcriptional activity. Multiple transcript variants encoding different isoforms have been found for this gene.
Western blot analysis of extracts of various cell lines, using RBFOX2 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.Western blot analysis of extracts of various cell lines, using RBFOX2 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
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