Antibody Name:	Anti-RBBP8 Antibody
Antibody SKU:	CAB10201
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human RBBP8 (NP_976036.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, HT-29, Mouse spleen, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human RBBP8 (NP_976036.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MNIS GSSC GSPN SADT SSDF KDLW TKLK ECHD REVQ GLQV KVTK LKQE RILD AQRL EEFF TKNQ QLRE QQKV LHET IKVL EDRL RAGL CDRC AVTE EHMR KKQQ EFEN IRQQ NLKL ITEL MNER NTLQ EENK KLSE QLQQ KIEN DQQH QAAE LECE EDVI PDSP ITAF SFSG VNRL RRKE NPHV RYIE QTHT KLEH SVCA NEMR KVSK SSTH PQHN PNEN EILV ADTY DQSQ SPMA KAHG TSSY TPDK SSFN LATV VAET LGLG VQEE SETQ GPMS PLGD ELYH CLEG NHKK QPFE ESTR
Gene ID:	5932
Uniprot:	Q99708
Cellular Location:	Nucleus
Calculated MW:	98kDa/101kDa/102kDa
Observed MW:	110kDa

Synonyms:

RBBP8, COM1, CTIP, JWDS, RIM, SAE2, SCKL2

Background:

The protein encoded by this gene is a ubiquitously expressed nuclear protein. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. This protein complexes with transcriptional co-repressor CTBP. It is also associated with BRCA1 and is thought to modulate the functions of BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. It is suggested that this gene may itself be a tumor suppressor acting in the same pathway as BRCA1. Three transcript variants encoding two different isoforms have been found for this gene. More transcript variants exist, but their full-length natures have not been determined.

UniProt Protein Function:	CtIP: a transcription factor containing C2H2 zinc fingers. May modulate the functions ascribed to BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. Interacts with CTBP, with the C-terminal (BRCT) domains of BRCA1, and with the retinoblastoma protein. Abundantly expressed in brain and the immune system and associated with immune system malignancies. Ionizing radiation induces its hyperphosphorylation and dissociation from BRCA1 in an ATM-dependent manner.
UniProt Protein Details:	Protein type:Transcription, coactivator/corepressor; EC 3.1.-.- Chromosomal Location of Human Ortholog: 18q11.2 Cellular Component: nucleolus; nucleoplasm; nucleus; transcriptional repressor complex Molecular Function:damaged DNA binding; protein binding; single-stranded DNA specific endodeoxyribonuclease activity Biological Process: cell cycle checkpoint; DNA double-strand break processing; DNA repair; DNA replication; DNA synthesis during DNA repair; double-strand break repair via homologous recombination; G2/M transition DNA damage checkpoint; nucleotide-excision repair; regulation of transcription from RNA polymerase II promoter; strand displacement Disease: Jawad Syndrome; Seckel Syndrome 2
NCBI Summary:	The protein encoded by this gene is a ubiquitously expressed nuclear protein. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. This protein complexes with transcriptional co-repressor CTBP. It is also associated with BRCA1 and is thought to modulate the functions of BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. It is suggested that this gene may itself be a tumor suppressor acting in the same pathway as BRCA1. Three transcript variants encoding two different isoforms have been found for this gene. More transcript variants exist, but their full-length natures have not been determined. [provided by RefSeq, Jul 2008]
UniProt Code:	Q99708
NCBI GenInfo Identifier:	116242745
NCBI Gene ID:	5932
NCBI Accession:	Q99708.2
UniProt Secondary Accession:	Q99708,O75371, Q8NHQ3, A6NKN2, A8K8W6, E7ETY1,
UniProt Related Accession:	Q99708
Molecular Weight:	98,434 Da
NCBI Full Name:	DNA endonuclease RBBP8
NCBI Synonym Full Names:	RB binding protein 8, endonuclease
NCBI Official Symbol:	RBBP8
NCBI Official Synonym Symbols:	RIM; COM1; CTIP; JWDS; SAE2; SCKL2
NCBI Protein Information:	DNA endonuclease RBBP8
UniProt Protein Name:	DNA endonuclease RBBP8
UniProt Synonym Protein Names:	CtBP-interacting protein; CtIP; Retinoblastoma-binding protein 8; RBBP-8; Retinoblastoma-interacting protein and myosin-like; RIM; Sporulation in the absence of SPO11 protein 2 homolog; SAE2
Protein Family:	RBBP8 N-terminal-like protein
UniProt Gene Name:	RBBP8
UniProt Entry Name:	COM1_HUMAN

	Western blot analysis of extracts of various cell lines, using RBBP8 antibody (CAB10201) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Western blot analysis of extracts of various cell lines, using RBBP8 antibody (CAB10201) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.