Developmental Biology

Anti-RASAL1 Antibody (CAB15114)

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SKU:
CAB15114
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Developmental Biology

Description

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Antibody Name:Anti-RASAL1 Antibody
Antibody SKU:CAB15114
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 120-340 of human RASAL1 (NP_004649.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:A549, MCF7, mouse brain, rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 120-340 of human RASAL1 (NP_004649.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ICLS VQML EDGQ GRCL RCHV LQAR DLAP RDIS GTSD PFAR VFWG SQSL ETST IKKT RFPH WDEV LELR EMPG APSP LRVE LWDW DMVG KNDF LGMV EFSP KTLQ QKPP KGWF RLLP FPRA EEDS GGNL GALR VKVR LIED RVLP SQCY QPLM ELLM ESVQ GPAE EDTA SPLA LLEE LTLG DCRQ DLAT KLVK LFLG RGLA GRFL DYLT RREV ARTM DPNT L
Gene ID:8437
Uniprot:O95294
Cellular Location:
Calculated MW:86kDa/90kDa
Observed MW:110kDa
Synonyms:RASAL1, RASAL
Background:The protein encoded by this gene is member of the GAP1 family of GTPase-activating proteins. These proteins stimulate the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. This particular family member contains domains which are characteristic of the GAP1 subfamily of RasGAP proteins but, in contrast to the other GAP1 family members, this protein is strongly and selectively expressed in endocrine tissues. Alternatively spliced transcript variants that encode different isoforms have been described
UniProt Protein Function:RASAL1: a GTPase activating protein for Ras. Negatively regulates Ras (H-Ras) signaling activity. Two alternatively-spliced isoforms have been described. PITX1 inhibits tumorigenicity of the Ras pathway by inducing the transcription of RASAL1, which in turn inactivates Ras. Two alternatively-spliced isoforms have been described.
UniProt Protein Details:

Protein type:GAPs, Ras; GAPs

Chromosomal Location of Human Ortholog: 12q23-q24

Cellular Component: cytosol; plasma membrane

Molecular Function:phospholipid binding

Biological Process: MAPKKK cascade; negative regulation of Ras protein signal transduction; signal transduction

NCBI Summary:The protein encoded by this gene is member of the GAP1 family of GTPase-activating proteins. These proteins stimulate the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. This particular family member contains domains which are characteristic of the GAP1 subfamily of RasGAP proteins but, in contrast to the other GAP1 family members, this protein is strongly and selectively expressed in endocrine tissues. Alternatively spliced transcript variants that encode different isoforms have been described [provided by RefSeq, Jul 2010]
UniProt Code:O95294
NCBI GenInfo Identifier:311033542
NCBI Gene ID:8437
NCBI Accession:O95294.3
UniProt Secondary Accession:O95294,Q52M03, Q59H24, Q96CC7, B7ZKM4, C9JFK5, F8VQX1
UniProt Related Accession:O95294
Molecular Weight:90,145 Da
NCBI Full Name:RasGAP-activating-like protein 1
NCBI Synonym Full Names:RAS protein activator like 1
NCBI Official Symbol:RASAL1  
NCBI Official Synonym Symbols:RASAL  
NCBI Protein Information:rasGAP-activating-like protein 1
UniProt Protein Name:RasGAP-activating-like protein 1
UniProt Synonym Protein Names:RAS protein activator like 1
Protein Family:RasGAP-activating-like protein
UniProt Gene Name:RASAL1  
UniProt Entry Name:RASL1_HUMAN
Anti-RASAL1 Antibody (CAB15114)Western blot analysis of extracts of various cell lines, using RASAL1 antibody (CAB15114) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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