Anti-Raptor Antibody (CAB20984)
- SKU:
- CAB20984
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
Description
| Antibody Name: | Anti-Raptor Antibody (CAB20984) |
| Antibody SKU: | CAB20984 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting, Immunofluorescence |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 888-1013 of human Raptor (NP_065812.1). |
| Application: | Western blotting, Immunofluorescence |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Rat |
| Positive Samples: | 293T, PC-3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 888-1013 of human Raptor (NP_065812.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LTND VAKQ PVSR DLPS GRPG TTGP AGAQ YTPH SHQF PRTR KMFD KGPE QTAD DADD AAGH KSFI SATV QTGF CDWS ARYF AQPV MKIP EEHD LESQ IRKE REWR FLRN SRVR RQAQ QVIQ KGIT RL |
| Cellular Location: | Cytoplasm, Cytoplasmic granule, Lysosome |
| Calculated MW: | 149kDa |
| Observed MW: | 150KDa |
| Synonyms: | KOG1, Mip1 |
| Background: | This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene. |
 | Immunofluorescence analysis of PC-12 using Raptor antibody at dilution of 1:100/1:800/1:3200. Blue: DAPI for nuclear staining. |
 | Western blot analysis of extracts of various cell lines, using Raptor antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s. |
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