Immunology Antibodies 2

Anti-Raptor Antibody (CAB16309)

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SKU:
CAB16309
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-Raptor Antibody
Antibody SKU:CAB16309
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant protein of human RPTOR
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:293T
Immunogen:Recombinant protein of human RPTOR
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:57521
Uniprot:Q8N122
Cellular Location:Cytoplasm, Cytoplasmic granule, Lysosome
Calculated MW:43kDa/131kDa/149kDa
Observed MW:140-150kDa
Synonyms:KOG1, Mip1, RPTOR
Background:This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:Raptor: Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1- mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Interacts with MTOR. Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR, AKT1S1/PRAS40 and DEPTOR. mTORC1 binds to and is inhibited by FKBP12-rapamycin. Binds directly to 4EBP1 and RPS6KB1 independently of its association with MTOR. Binds preferentially to poorly or non-phosphorylated forms of EIF4EBP1, and this binding is critical to the ability of MTOR to catalyze phosphorylation. Forms a complex with MTOR under both leucine-rich and -poor conditions. Interacts with ULK1 in a nutrient-dependent manner; the interaction is reduced during starvation. Interacts (when phosphorylated by AMPK) with 14-3-3 protein, leading to inhibit its activity. Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta. Belongs to the WD repeat RAPTOR family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: cytoplasm; cytosol; intracellular membrane-bound organelle; lysosomal membrane; lysosome; nucleoplasm

Molecular Function:protein binding; protein binding, bridging; protein complex binding; protein kinase activator activity; protein kinase binding

Biological Process: cell cycle arrest; cell growth; cellular response to nutrient levels; macroautophagy; positive regulation of TOR signaling pathway; positive regulation of transcription from RNA polymerase III promoter; regulation of cell size; TOR signaling pathway

NCBI Summary:This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
UniProt Code:Q8N122
NCBI GenInfo Identifier:46577501
NCBI Gene ID:57521
NCBI Accession:Q8N122.1
UniProt Secondary Accession:Q8N122,Q8N4V9, Q8TB32, Q9P2P3, B2RN36, C6KEF2, F5H7J5
UniProt Related Accession:Q8N122
Molecular Weight:131,515 Da
NCBI Full Name:Regulatory-associated protein of mTOR
NCBI Synonym Full Names:regulatory associated protein of MTOR complex 1
NCBI Official Symbol:RPTOR  
NCBI Official Synonym Symbols:KOG1; Mip1  
NCBI Protein Information:regulatory-associated protein of mTOR
UniProt Protein Name:Regulatory-associated protein of mTOR
UniProt Synonym Protein Names:p150 target of rapamycin (TOR)-scaffold protein
Protein Family:Regulatory-associated protein
UniProt Gene Name:RPTOR  
UniProt Entry Name:RPTOR_HUMAN
Anti-Raptor Antibody (CAB16309)Western blot analysis of extracts of 293T cells, using Raptor antibody (CAB16309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5min.
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