Name: Anti-RALBP1 Antibody (CAB1140)[KO Validated]
Brand: KO Validated Antibodies 1
SKU: CAB1140
Availability: OutOfStock

Antibody Name:	Anti-RALBP1 Antibody [KO Validated]
Antibody SKU:	CAB1140
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 396-655 of human RALBP1 (NP_006779.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-673, Mouse spleen, Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 396-655 of human RALBP1 (NP_006779.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AGIK EEIR RQEF LLNC LHRD LQGG IKDL SKEE RLWE VQRI LTAL KRKL REAK RQEC ETKI AQEI ASLS KEDV SKEE MNEN EEVI NILL AQEN EILT EQEE LLAM EQFL RRQI ASEK EEIE RLRA EIAE IQSR QQHG RSET EEYS SESE SESE DEEE LQII LEDL QRQN EELE IKNN HLNQ AIHE EREA IIEL RVQL RLLQ MQRA KAEQ QAQE DEEP EWRG GAVQ PPRD GVLE PKAA KEQP KAGK EPAK PSPS RDRK ETSI
Gene ID:	10928
Uniprot:	Q15311
Cellular Location:	Membrane, Peripheral membrane protein
Calculated MW:	76kDa
Observed MW:	100kDa

Synonyms:	RALBP1, RIP1, RLIP1, RLIP76
Background:

UniProt Protein Function:	RALBP1: Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDK1 to switch off endocytosis, One of its substrates would be EPN1/Epsin. Interacts with the GTP-bound form of RALA, RALB, CDC42 and RAC1. Interacts with REPS1 and REPS2 and this does not affect the Ral-binding activity. Interacts with DAB2IP. Interacts with catalytically active CCNB1 and CDK1 during mitosis. Interacts with EPN1, NUMB and TFAP2A during interphase and mitosis. Expressed ubiquitously but at low levels. Shows a strong expression in the erythrocytes.
UniProt Protein Details:	Protein type:GAPs; GAPs, Rac/Rho Chromosomal Location of Human Ortholog: 18p11.3 Cellular Component: membrane; cytosol Molecular Function:protein binding; ATPase activity, coupled to movement of substances; ATPase activity; Rac GTPase binding; Ral GTPase binding; GTPase activator activity Biological Process: regulation of GTPase activity; regulation of small GTPase mediated signal transduction; metabolic process; transport; small GTPase mediated signal transduction; chemotaxis; signal transduction
NCBI Summary:	RALBP1 plays a role in receptor-mediated endocytosis and is a downstream effector of the small GTP-binding protein RAL (see RALA; MIM 179550). Small G proteins, such as RAL, have GDP-bound inactive and GTP-bound active forms, which shift from the inactive to the active state through the action of RALGDS (MIM 601619), which in turn is activated by RAS (see HRAS; MIM 190020) (summary by Feig, 2003 [PubMed 12888294]).[supplied by OMIM, Nov 2010]
UniProt Code:	Q15311
NCBI GenInfo Identifier:	34098413
NCBI Gene ID:	10928
NCBI Accession:	Q15311.3
UniProt Related Accession:	Q15311
Molecular Weight:
NCBI Full Name:	RalA-binding protein 1
NCBI Synonym Full Names:	ralA binding protein 1
NCBI Official Symbol:	RALBP1
NCBI Official Synonym Symbols:	RIP1; RLIP1; RLIP76
NCBI Protein Information:	ralA-binding protein 1
UniProt Protein Name:	RalA-binding protein 1
UniProt Synonym Protein Names:	76 kDa Ral-interacting protein; Dinitrophenyl S-glutathione ATPase; DNP-SG ATPase; Ral-interacting protein 1
Protein Family:	RNA-binding protein
UniProt Gene Name:	RALBP1
UniProt Entry Name:	RBP1_HUMAN

	Western blot analysis of extracts of various cell lines, using RALBP1 antibody (CAB1140) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Western blot analysis of extracts from normal (control) and RALBP1 knockout (KO) HeLa cells, using RALBP1 antibody (CAB1140) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded rat spleen using RALBP1 antibody (CAB1140) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat heart using RALBP1 antibody (CAB1140) at dilution of 1:100 (40x lens).