Epigenetics & Nuclear Signaling Antibodies 3

Anti-Rad51D Antibody (CAB4068)

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SKU:
CAB4068
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-Rad51D Antibody
Antibody SKU:CAB4068
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-328 of human Rad51D (NP_002869.3).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HL-60, Jurkat, HT-29, Mouse liver, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-328 of human Rad51D (NP_002869.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGVL RVGL CPGL TEEM IQLL RSHR IKTV VDLV SADL EEVA QKCG LSYK ALVA LRRV LLAQ FSAF PVNG ADLY EELK TSTA ILST GIGS LDKL LDAG LYTG EVTE IVGG PGSG KTQV CLCM AANV AHGL QQNV LYVD SNGG LTAS RLLQ LLQA KTQD EEEQ AEAL RRIQ VVHA FDIF QMLD VLQE LRGT VAQQ VTGS SGTV KVVV VDSV TAVV SPLL GGQQ REGL ALMM QLAR ELKT LARD LGMA VVVT NHIT RDRD SGRL KPAL GRSW SFVP STRI LLDT IEGA GASG GRRM ACLA KSSR QPTG FQEM VDIG TWGT SEQS ATLQ GDQT
Gene ID:5892
Uniprot:O75771
Cellular Location:Chromosome, Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center, telomere
Calculated MW:5kDa/12kDa/22kDa/23kDa/30kDa/35kDa/37kDa
Observed MW:35kDa
Synonyms:RAD51D, BROVCA4, R51H3, RAD51L3, TRAD, Rad51D
Background:The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, which are known to be involved in the homologous recombination and repair of DNA. This protein forms a complex with several other members of the RAD51 family, including RAD51L1, RAD51L2, and XRCC2. The protein complex formed with this protein has been shown to catalyze homologous pairing between single- and double-stranded DNA, and is thought to play a role in the early stage of recombinational repair of DNA. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the downstream ring finger and FYVE-like domain containing 1 (RFFL) gene.
UniProt Protein Function:RAD51L3: Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. The BCDX2 complex binds single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. Defects in RAD51D are a cause of susceptibility to familial breast-ovarian cancer type 4 (BROVCA4). A condition associated with familial predisposition to cancer of the breast and ovaries. Characteristic features in affected families are an early age of onset of breast cancer (often before age 50), increased chance of bilateral cancers (cancer that develop in both breasts, or both ovaries, independently), frequent occurrence of breast cancer among men, increased incidence of tumors of other specific organs, such as the prostate. Belongs to the RecA family. RAD51 subfamily. 8 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 17q11

Cellular Component: centrosome; chromosome, telomeric region; cytoplasm; nuclear chromosome, telomeric region; nucleoplasm; nucleus; replication fork

Molecular Function:ATP binding; DNA binding; DNA-dependent ATPase activity; double-stranded DNA binding; endodeoxyribonuclease activity; four-way junction DNA binding; gamma-tubulin binding; protein binding; recombinase activity; single-stranded DNA binding

Biological Process: DNA repair; double-strand break repair; double-strand break repair via homologous recombination; double-strand break repair via synthesis-dependent strand annealing; meiotic DNA recombinase assembly; meiotic recombination; mitotic recombination; nucleotide-excision repair; regulation of cell cycle; response to ionizing radiation; strand invasion; telomere maintenance

Disease: Breast-ovarian Cancer, Familial, Susceptibility To, 4

NCBI Summary:The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, which are known to be involved in the homologous recombination and repair of DNA. This protein forms a complex with several other members of the RAD51 family, including RAD51L1, RAD51L2, and XRCC2. The protein complex formed with this protein has been shown to catalyze homologous pairing between single- and double-stranded DNA, and is thought to play a role in the early stage of recombinational repair of DNA. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the downstream ring finger and FYVE-like domain containing 1 (RFFL) gene. [provided by RefSeq, Jan 2011]
UniProt Code:O75771
NCBI GenInfo Identifier:6174940
NCBI Gene ID:5892
NCBI Accession:O75771.1
UniProt Secondary Accession:O75771,O43537, O60355, O75196, O75847, O75848, O76073 O76085, O94908, Q9UFU5, B4DJU7, E1P637,
UniProt Related Accession:O75771
Molecular Weight:37,014 Da
NCBI Full Name:DNA repair protein RAD51 homolog 4
NCBI Synonym Full Names:RAD51 paralog D
NCBI Official Symbol:RAD51D  
NCBI Official Synonym Symbols:TRAD; R51H3; BROVCA4; RAD51L3  
NCBI Protein Information:DNA repair protein RAD51 homolog 4
UniProt Protein Name:DNA repair protein RAD51 homolog 4
UniProt Synonym Protein Names:R51H3; RAD51 homolog D; RAD51-like protein 3; TRAD
Protein Family:DNA repair protein
UniProt Gene Name:RAD51D  
UniProt Entry Name:RA51D_HUMAN
Anti-Rad51D Antibody (CAB4068)Western blot analysis of extracts of various cell lines, using RAD51D antibody (CAB4068) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-Rad51D Antibody (CAB4068)Immunofluorescence analysis of A549 cells using RAD51D antibody (CAB4068). Blue: DAPI for nuclear staining.
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