Antibody Name:	Anti-Rad50 Antibody
Antibody SKU:	CAB3078
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Rad50 (NP_005723.2).

Application:	WB IHC IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:20 - 1:50
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, MCF7, 293T, HepG2, NIH/3T3, Mouse testis, Mouse spleen, Mouse thymus, Rat testis

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Rad50 (NP_005723.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSRI EKMS ILGV RSFG IEDK DKQI ITFF SPLT ILVG PNGA GKTT IIEC LKYI CTGD FPPG TKGN TFVH DPKV AQET DVRA QIRL QFRD VNGE LIAV QRSM
Gene ID:	10111
Uniprot:	Q92878
Cellular Location:	Chromosome, Nucleus, telomere
Calculated MW:	138kDa/153kDa/154kDa
Observed MW:	170kDa

Synonyms:

RAD50, NBSLD, RAD502, hRad50

Background:

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.

UniProt Protein Function:	Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point (PubMed:11741547, PubMed:9590181, PubMed:9705271, PubMed:9651580). The complex may also be required for DNA damage signaling via activation of the ATM kinase (PubMed:15064416). In telomeres the MRN complex may modulate t-loop formation (PubMed:10888888).
NCBI Summary:	The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.[provided by RefSeq, Apr 2010]
UniProt Code:	Q92878
NCBI GenInfo Identifier:	60392986
NCBI Gene ID:	10111
NCBI Accession:	Q92878.1
UniProt Secondary Accession:	Q92878,O43254, Q6GMT7, Q6P5X3, Q9UP86, B9EGF5,
UniProt Related Accession:	Q92878
Molecular Weight:	155 kDa
NCBI Full Name:	DNA repair protein RAD50
NCBI Synonym Full Names:	RAD50 double strand break repair protein
NCBI Official Symbol:	RAD50
NCBI Official Synonym Symbols:	NBSLD; RAD502; hRad50
NCBI Protein Information:	DNA repair protein RAD50
UniProt Protein Name:	DNA repair protein RAD50
Protein Family:	RAD50-interacting protein
UniProt Gene Name:	RAD50

	Western blot analysis of extracts of various cell lines, using Rad50 antibody (CAB3078) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human lung cancer using Rad50 antibody (CAB3078) at dilution of 1:100 (40x lens).
	Immunoprecipitation analysis of 150ug extracts of MCF7 cells using 3ug Rad50 antibody (CAB3078). Western blot was performed from the immunoprecipitate using Rad50 antibody (CAB3078) at a dilition of 1:500.